A poly-l-lysine-bonded TEMPO-oxidized bacterial nanocellulose-based antibacterial dressing for infected wound treatment

Mina Shahriari-Khalaji; Geli Li; Lu Liu; Mamoona Sattar; Lin Chen; Chunyan Zhong; Feng F Hong

doi:10.1016/j.carbpol.2022.119266

A poly-l-lysine-bonded TEMPO-oxidized bacterial nanocellulose-based antibacterial dressing for infected wound treatment

Carbohydr Polym. 2022 Jul 1:287:119266. doi: 10.1016/j.carbpol.2022.119266. Epub 2022 Feb 24.

Authors

Mina Shahriari-Khalaji¹, Geli Li¹, Lu Liu¹, Mamoona Sattar¹, Lin Chen¹, Chunyan Zhong², Feng F Hong³

Affiliations

¹ Microbiological Engineering and Industrial Biotechnology Group, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, Shanghai 201620, China.
² Hainan Guangyu Biotechnology Co., Ltd., Haikou 570100, China.
³ Microbiological Engineering and Industrial Biotechnology Group, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, Shanghai 201620, China; Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Donghua University, Shanghai 201620, China. Electronic address: fhong@dhu.edu.cn.

PMID: 35422281
DOI: 10.1016/j.carbpol.2022.119266

Abstract

Oxidized bacterial nanocellulose (O-BNC) is a favorable material to subdue bacterial infection because of the carboxylate content that not only has a weak antibacterial activity but also is capable of bonding electrostatically to polycationic antibacterial agents. In this study, the 2,2,6,6-Tetramethylpiperidinyloxy radical (TEMPO)-mediated oxidation of BNC was optimized to achieve high carboxylate content while retaining an acceptable tensile profile. To develop an O-BNC-based functional wound dressing, ε-poly-l-lysine (PLL) was then covalently bonded with O-BNC via 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) reaction after homogeneous distribution by ultrasonication. The antibacterial activity of the obtained wound dressing was significantly higher (p < 0.05), and no toxicity was observed. The infected full-thickness wounds of rats were healed faster (p < 0.05) covered by the dressing due to less inflammation, faster blood vessel proliferation, and epidermal layer formation. The material is an effective and promising functional dressing for the treatment of infected wounds.

Keywords: Antibacterial wound dressing; Bacterial nanocellulose; TEMPO-oxidation; Ultrasonication; ε-Poly-l-lysine.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacteria
Bandages
Cyclic N-Oxides
Polylysine*
Rats
Wound Infection* / drug therapy

Substances

Anti-Bacterial Agents
Cyclic N-Oxides
Polylysine
TEMPO