Development of Optical Biosensor for the Detection of Glutamine in Human Biofluids Using Merocyanine Dye

Vijayakumar Sathya; Appadurai Deepa; Lakshmi Kandhan Sangeetha; Venkatesan Srinivasadesikan; Shyi-Long Lee; Vediappen Padmini

doi:10.1007/s10895-022-02937-y

Development of Optical Biosensor for the Detection of Glutamine in Human Biofluids Using Merocyanine Dye

J Fluoresc. 2022 Jul;32(4):1389-1396. doi: 10.1007/s10895-022-02937-y. Epub 2022 Apr 14.

Authors

Vijayakumar Sathya¹, Appadurai Deepa¹, Lakshmi Kandhan Sangeetha¹, Venkatesan Srinivasadesikan², Shyi-Long Lee³, Vediappen Padmini⁴

Affiliations

¹ Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India.
² Division of Chemistry, Department of Sciences and Humanities, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Guntur, 522 213, Andhra Pradesh, India.
³ Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi, 621, Taiwan.
⁴ Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India. padmini.chem@mkuniversity.org.

PMID: 35420326
DOI: 10.1007/s10895-022-02937-y

Abstract

Merocyanine dye based fluorescent organic compound has been synthesized for the detection of glutamine. The probe showed remarkable fluorescent intensity with glutamine through ICT (Intermolecular Charge Transfer Mechanism). Hence, it is tested for the detection of glutamine using colorimetric and fluorimetric techniques in physiological and neutral pH (7.2). Under optimized experimental conditions, the probe detects glutamine selectively among other interfering biomolecules. The probe has showed a LOD (lower limit of detection) of 9.6 × 10^-8 mol/L at the linear range 0-180 µM towards glutamine. The practical application of the probe is successfully tested in human biofluids.

Keywords: Competitive study; Fluorimetry biosensor; Glutamine; LOD.

MeSH terms

Biosensing Techniques* / methods
Fluorescent Dyes* / chemistry
Glutamine
Humans
Limit of Detection
Pyrimidinones

Substances

Fluorescent Dyes
Pyrimidinones
Glutamine
merocyanine dye