Structure-based redesign of Fe(II)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation

Takahiro Mori; Yu Nakashima; Heping Chen; Shotaro Hoshino; Takaaki Mitsuhashi; Ikuro Abe

doi:10.1039/d2cc00736c

Structure-based redesign of Fe(II)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation

Chem Commun (Camb). 2022 May 3;58(36):5510-5513. doi: 10.1039/d2cc00736c.

Authors

Takahiro Mori^{1

2

3}, Yu Nakashima¹, Heping Chen¹, Shotaro Hoshino¹, Takaaki Mitsuhashi¹, Ikuro Abe^{1

2}

Affiliations

¹ Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan. tmori@mol.f.u-tokyo.ac.jp.
² Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
³ PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan.

PMID: 35420093
DOI: 10.1039/d2cc00736c

Abstract

Structure- and mechanism-based redesign of the Fe(II)/2-oxoglutarate-dependent oxygenase AndA was performed. The function of AndA was expanded to catalyze a spiro-ring formation reaction from an isomerization reaction. The redesigned AndA variants produced two unnatural novel spiro-ring containing compounds through two and three consecutive oxidation reactions.

MeSH terms

Catalysis
Ferrous Compounds
Ketoglutaric Acids*
Oxidation-Reduction
Oxygenases* / metabolism

Substances

Ferrous Compounds
Ketoglutaric Acids
Oxygenases