The aim of this study was to determine the CK activity and its association with the length of hospital stay in acutely intoxicated patients with psychotropic and chemical substances. Rhabdomyolysis is defined as a creatine kinase (CK) > 250 U/L. We included adult patients ≥ 18 years of age, with rhabdomyolysis acutely intoxicated with psychotropic and chemical substances in the first 48 hours. We excluded patients with rhabdomyolysis in muscle trauma as a result of a traumatic accident, myocardial infarction, cerebral vascular infarction, cerebral hemorrhage, and chronic hepatic and renal disease. In all patients with rhabdomyolysis, the mean CK on the first, third and fifth day was consequently 5715.9±16088.8 U/L with a maximum value of 129077 U/L vs. 5548.5±9851.5 U/L with a maximum value of 63947U/L vs. 2970.42±7161.68 U/L with a maximum value of 53672 U/L. The comparison for the whole sample in the three measurement times, for p <0.05, showed a significant difference (Friedman Test: N = 62; Chi-Square = 34.935; df = 2; p = 0.00001). For the whole sample of patients with rhabdomyolysis, as well as in the group of psychotropic intoxications, for p < 0.05, the level of CK on the first day was confirmed as an independent predictor that significantly affected the variability of the length of hospitalization by 5.2% (R2 = 0.052) vs. 20% (R2=0.200). In rhabdomyolysis, as a result of acute intoxication with psychotropic and chemical substances it is important to examine the creatine kinase. Creatine kinase levels were higher in rhabdomyolysis in acutely intoxicated patients with psychoactive compared to chemical substances. In the group intoxicated with psychoactive substances creatine kinase as an independent predictor significantly affected the length of hospitalization.