Real-time quantitative PCR method for detection and quantification of Clostridioides difficile cells and spores

J Microbiol Methods. 2022 May:196:106458. doi: 10.1016/j.mimet.2022.106458. Epub 2022 Apr 11.

Abstract

A quantitative PCR method was developed for the detection of Clostridioides difficile Chaperonin-60 gene. The method had a specificity for C. difficile strains of clinical and epidemiological importance and allowed for quantification of C. difficile cells and spores.

Keywords: Clostridioides difficile; Pathogen detection; Spores; qPCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clostridioides difficile* / genetics
  • Clostridioides*
  • Real-Time Polymerase Chain Reaction / methods
  • Spores, Bacterial / genetics