The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action

Josy Goldoni Lazarini; Adna Prado Massarioli; Jackeline Cintra Soares; Bruno Dias Nani; Nancy Charo; Douglas Souza Oliveira; Lauren Camargo; Miryam Paola Alvarez-Flores; Isabel de Fátima Correia Batista; Ana Marisa Chudzinski-Tavassi; Severino Matias de Alencar; Marcelo Franchin; Pedro Luiz Rosalen

doi:10.1016/j.fochms.2022.100093

The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action

Food Chem (Oxf). 2022 Mar 7:4:100093. doi: 10.1016/j.fochms.2022.100093. eCollection 2022 Jul 30.

Authors

Josy Goldoni Lazarini¹, Adna Prado Massarioli², Jackeline Cintra Soares², Bruno Dias Nani¹, Nancy Charo³, Douglas Souza Oliveira⁴, Lauren Camargo⁴, Miryam Paola Alvarez-Flores⁴, Isabel de Fátima Correia Batista⁴, Ana Marisa Chudzinski-Tavassi⁴, Severino Matias de Alencar², Marcelo Franchin^{1

5}, Pedro Luiz Rosalen^{1

6}

Affiliations

¹ Department of Biosciences, Piracicaba Dental School, University of Campinas, Limeira Avenue, 901, Areião, 13414-903 Piracicaba, SP, Brazil.
² Department of Agri-Food Industry, Food and Nutrition, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Pádua Dias Avenue, P.O. Box. 9, 13418-900 Piracicaba, SP, Brazil.
³ Laboratory of Experimental Thrombosis, Institute of Experimental Medicine, CONICET, National Academy of Medicine, 1425 Buenos Aires, Argentina.
⁴ Centre of Excellence in New Target Discovery (CENTD), Development and Innovation Laboratory, Butantan Institute, São Paulo, SP, Brazil.
⁵ Faculty of Dentistry, Federal University of Alfenas, Alfenas, MG, Brazil.
⁶ Biological Sciences Graduate Program, Federal University of Alfenas, Alfenas, MG, Brazil.

Abstract

We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.

Keywords: Anti-inflammatory; Eugenia selloi; Native fruit; ROS and RNS.