Designing CoS1.035 Nanoparticles Anchored on N-Doped Carbon Dodecahedron as Dual-Enzyme Mimics for the Colorimetric Detection of H2O2 and Glutathione

Tao Chen; Yuanhong Min; Xiao Yang; Hao Gong; Xiaoying Tian; Li Liu; Yanhua Hou; Wensheng Fu

doi:10.1021/acsomega.1c07264

Designing CoS_1.035 Nanoparticles Anchored on N-Doped Carbon Dodecahedron as Dual-Enzyme Mimics for the Colorimetric Detection of H₂O₂ and Glutathione

ACS Omega. 2022 Mar 24;7(13):11135-11147. doi: 10.1021/acsomega.1c07264. eCollection 2022 Apr 5.

Authors

Tao Chen¹, Yuanhong Min¹, Xiao Yang¹, Hao Gong¹, Xiaoying Tian¹, Li Liu¹, Yanhua Hou², Wensheng Fu¹

Affiliations

¹ Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
² Chongqing Engineering Research Center of Pharmaceutical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China.

Abstract

In recent years, the exploration of the nanozyme, an artificial enzyme with the structure and function of natural enzymes, has become a hot topic in this field. Although significant progress has been made, it is still a huge challenge to design nanozymes with multiple enzyme-like catalytic activities. In this work, we have successfully fabricated a colorimetric sensing platform to mimic peroxidase-like and oxidase-like activities by the CoS_1.035 nanoparticles decorated N-doped carbon framework porous dodecahedrons (abbreviated to CoS_1.035/N-C PDHs). And the catalytic mechanism of CoS_1.035/N-C PDHs toward the peroxidase-like and oxidase-like activities is systematically explored. The results display that CoS_1.035/N-C PDHs can catalyze the oxidation of the colorless substrate 3,3,'5,5'-tetramethylbenzidine (TMB) into blue oxidized TMB (ox-TMB) by disintegrating H₂O₂ or the physically/chemically absorbed O₂ into different ROS species (·OH or O₂ ^·-) in the presence or absence of H₂O₂. Therefore, on the basis of the dual-enzyme mimic activities of CoS_1.035/N-C PDHs, the bifunctional colorimetric sensing platform is established for H₂O₂ detection with a wide linear range of 0.5-120 μM and glutathione detection with a linear range of 1-60 μM, respectively. This work provides an efficient platform for dual-enzyme mimics, expanding the application prospect of Co-based chalcogenides as enzyme mimics in biosensing, medical diagnosis, and environment monitoring.