Wood vinegar facilitated growth and Cd/Zn phytoextraction of Sedum alfredii Hance by improving rhizosphere chemical properties and regulating bacterial community

Xueqi Zhou; An Shi; Christopher Rensing; Jing Yang; Wuzhong Ni; Shihe Xing; Wenhao Yang

doi:10.1016/j.envpol.2022.119266

Wood vinegar facilitated growth and Cd/Zn phytoextraction of Sedum alfredii Hance by improving rhizosphere chemical properties and regulating bacterial community

Environ Pollut. 2022 Jul 15:305:119266. doi: 10.1016/j.envpol.2022.119266. Epub 2022 Apr 9.

Authors

Xueqi Zhou¹, An Shi¹, Christopher Rensing¹, Jing Yang¹, Wuzhong Ni², Shihe Xing¹, Wenhao Yang³

Affiliations

¹ Key Laboratory of Soil Ecosystem Health and Regulation of Fujian Provincial University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
² College of Environment and Resources, Zhejiang University, Hangzhou, 310058, China.
³ Key Laboratory of Soil Ecosystem Health and Regulation of Fujian Provincial University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; College of Environment and Resources, Zhejiang University, Hangzhou, 310058, China. Electronic address: whyang@fafu.edu.cn.

PMID: 35413404
DOI: 10.1016/j.envpol.2022.119266

Abstract

Soil Cd and Zn contamination has become a serious environmental problem. This work explored the performance of wood vinegar (WV) in enhancing the phytoextraction of Cd/Zn by hyperaccumulator Sedum alfredii Hance. Rhizosphere chemical properties, enzyme activities and bacterial community were analyzed to determine the mechanisms of metal accumulation in this process. Results demonstrated that, after 120 days growth, different times dilution of WV increased the shoot biomass of S. alfredii by 85.2%-148%. In addition, WV application significantly increased soil available Cd and Zn by lowing soil pH, which facilitated plant uptake. The optimal Cd and Zn phytoextraction occurred from the 100 times diluted WV (D100), which increased the Cd and Zn extraction by 188% and 164%, compared to CK. The 100 and 50 times diluted WV significantly increased soil total and available carbon, nitrogen and phosphorus, and enhancing enzyme activities of urease, acid phosphatase, invertase and protease by 10.1-21.4%, 29.1-42.7%,12.2-38.3% and 26.8-85.7%, respectively, compared to CK. High-throughput sequencing revealed that the D 100 significantly increased the bacterial diversity compared to CK. Soil bacterial compositions at phylum, family and genera level were changed by WV addition. Compared to CK, WV application increased the relative abundances of genus with plant growth promotion and metal mobilization function such as, Bacillus, Gemmatimonas, Streptomyces, Sphingomonas and Polycyclovorans, which was positively correlated to biomass, Cd/Zn concentrations and extractions by S. alfredii. Structural equation modeling analysis showed that, soil chemical properties, enzyme activities and bacterial abundance directly or indirectly contributed to the biomass promotion, Cd, and Zn extraction by S. alfredii. To sum up, WV improved phytoextraction efficiency by enhancing plant growth, Cd and Zn extraction and increasing soil nutrients, enzyme activities, and modifying bacterial community.

Keywords: Bacterial community; Phytoextraction; Rhizosphere; Sedum alfredii Hance; Wood vinegar.

MeSH terms

Acetic Acid
Bacteria / metabolism
Biodegradation, Environmental
Cadmium / analysis
Methanol
Plant Roots / metabolism
Rhizosphere
Sedum* / metabolism
Sedum* / microbiology
Soil / chemistry
Soil Pollutants* / analysis
Zinc / analysis

Substances

Soil
Soil Pollutants
Cadmium
wood vinegar
Zinc
Acetic Acid
Methanol