A new membrane formulation for modelling the flow of stomatocyte, discocyte, and echinocyte red blood cells

D M W Karandeniya; D W Holmes; E Sauret; Y T Gu

doi:10.1007/s10237-022-01567-4

A new membrane formulation for modelling the flow of stomatocyte, discocyte, and echinocyte red blood cells

Biomech Model Mechanobiol. 2022 Jun;21(3):899-917. doi: 10.1007/s10237-022-01567-4. Epub 2022 Apr 12.

Authors

D M W Karandeniya¹, D W Holmes², E Sauret³, Y T Gu³

Affiliations

¹ School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, Australia. dinushika.karandeniya@hdr.qut.edu.au.
² School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, Australia. d.holmes@qut.edu.au.
³ School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, Australia.

Abstract

In this work, a numerical model that enables simulation of the deformation and flow behaviour of differently aged Red Blood Cells (RBCs) is developed. Such cells change shape and decrease in deformability as they age, thus impacting their ability to pass through the narrow capillaries in the body. While the body filters unviable cells from the blood naturally, cell aging poses key challenges for blood stored for transfusions. Therefore, understanding the influence RBC morphology and deformability have on their flow is vital. While several existing models represent young Discocyte RBC shapes well, a limited number of numerical models are developed to model aged RBC morphologies like Stomatocytes and Echinocytes. The existing models are also limited to shear and stretching simulations. Flow characteristics of these morphologies are yet to be investigated. This paper aims to develop a new membrane formulation for the numerical modelling of Stomatocyte, Discocytes and Echinocyte RBC morphologies to investigate their deformation and flow behaviour. The model used represents blood plasma using the Lattice Boltzmann Method (LBM) and the RBC membrane using the discrete element method (DEM). The membrane and the plasma are coupled by the Immersed Boundary Method (IBM). Previous LBM-IBM-DEM formulations represent RBC membrane response based on forces generated from changes in the local area, local length, local bending, and cell volume. In this new model, two new force terms are added: the local area difference force and the local curvature force, which are specially incorporated to model the flow and deformation behaviour of Stomatocytes and Echinocytes. To verify the developed model, the deformation behaviour of the three types of RBC morphologies are compared to well-characterised stretching and shear experiments. The flow modelling capabilities of the method are then demonstrated by modelling the flow of each cell through a narrow capillary. The developed model is found to be as accurate as benchmark Smoothed Particle Hydrodynamics (SPH) approaches while being significantly more computationally efficient.

Keywords: Discocytes; Echinocytes; Lattice Boltzmann Method; Red Blood Cells; Stomatocytes.

MeSH terms

Cell Size
Computer Simulation
Erythrocyte Deformability*
Erythrocytes*
Hydrodynamics

Grants and funding

DP180103009/australian research council