Application of zinc oxide nanoparticles as fertilizer boosts growth in rice plant and alleviates chromium stress by regulating genes involved in oxidative stress

Abstract

Chromium toxicity impairs the productivity of rice crops and raises a major concern worldwide and thus, it calls for unconventional and sustainable means of crop production. In this study, we identified the implication of zinc oxide nanoparticles (ZnO NPs) in promoting plant growth and ameliorating chromium-induced stress in seedlings of rice (Oryza sativa). This investigation demonstrates that the exogenous supplementation of ZnO NPs at 25 μM activates defense mechanisms conferring rice seedlings significant tolerance against stress imposed by the exposure of 100 μM Cr(VI). Further, supplementation of this nanofertilizer reversed the inhibitory effects of Cr(VI) on growth and photosynthetic efficiency. The growth promotion was primarily associated with the function of ZnO NPs in inducing activity of antioxidative enzymes i.e. APX, DHAR, MDHAR and GR belonging to the ascorbate-glutathione cycle in the Cr-exposed seedlings, exceeding the levels in control. The overexpression of these antioxidative genes correlated concomitantly with the decrease of oxidants including SOR and H₂O₂ and the increase in the levels of non-enzymatic antioxidants: AsA and GSH.

Keywords: Antioxidative enzymes; Chromium; Gene expression; Growth; Photosynthetic efficiency; Zinc oxide nanoparticles (ZnO NPs).