Generation of human embryonic stem cell-derived lung organoids

Le Han; Shanshan Zhao; Fuxun Yu; Zhili Rong; Ying Lin; Yong Chen

doi:10.1016/j.xpro.2022.101270

Generation of human embryonic stem cell-derived lung organoids

STAR Protoc. 2022 Apr 4;3(2):101270. doi: 10.1016/j.xpro.2022.101270. eCollection 2022 Jun 17.

Authors

Le Han¹, Shanshan Zhao¹, Fuxun Yu², Zhili Rong¹, Ying Lin¹, Yong Chen²

Affiliations

¹ Cancer Research Institute, School of Basic Medical Sciences, State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Key Laboratory of Organ Failure Research (Ministry of Education), Southern Medical University, Guangzhou 510515, China.
² Central Laboratory, Guizhou Provincial People's Hospital, Guiyang 550002, China.

Abstract

This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provides a promising tool to study human lung development, drug screening, regeneration, and disease modeling in vitro. For complete details on the use and execution of this protocol, please refer to Chen et al. (2018).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Developmental biology; Organoids; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drug Evaluation, Preclinical
Epithelial Cells
Human Embryonic Stem Cells*
Humans
Lung
Organoids* / metabolism