Molecular markers dataset to assess the genetic diversity of oriental plane trees from historical sites in Lazio (central Italy)

Enrica Alicandri; Anna Maria Vettraino; Mariagrazia Agrimi; Mario Ciaffi; Elena Kuzminsky

doi:10.1016/j.dib.2022.108100

Molecular markers dataset to assess the genetic diversity of oriental plane trees from historical sites in Lazio (central Italy)

Data Brief. 2022 Mar 25:42:108100. doi: 10.1016/j.dib.2022.108100. eCollection 2022 Jun.

Authors

Enrica Alicandri¹, Anna Maria Vettraino¹, Mariagrazia Agrimi¹, Mario Ciaffi¹, Elena Kuzminsky¹

Affiliation

¹ Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, snc I-01100, Viterbo, Italy.

Abstract

The dataset presented in this article is related to the research paper titled "Dimensional and genetic characterization of the last oriental plane trees (Platanus orientalis L.) of historical sites in Lazio (central Italy)" (Ciaffi et al., 2022). Indeed, the molecular analyses reported in that article consisted in a comparison of Italian veteran plane trees with 12 certified accessions of P. orientalis, P. occidentalis and their hybrids P. acerifolia (4 individuals per species). First, LEAFY gene analyses allowed identifying 32 P. orientalis and two P. acerifolia in four sites of the province of Rome, confirming also that the two representative trees from the two gardens of the province of Viterbo belong to P. orientalis. Second, the use of Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) molecular markers provided useful information regarding the genetic relationships within and among all the historical sites. Owing to the use of SSR and ISSR molecular markers, a dataset of parameters related to the genetic diversity of the same plant material was obtained and presented in this article. For SSR markers, seven loci previously developed for P. occidentalis (Lang, 2010) and two specifically developed for P. orientalis (Rinaldi et al., 2019) were employed. For ISSR markers, DNA samples were amplified with eight primers before used for the determination of genetic stability of micro-propagated plantlets of P. acerifolia (Huang et al., 2009) and for the genetic characterization of plane trees within the formal gardens of Villa Lante of Bagnaia and Palazzo Farnese (Viterbo, Italy) (Ciaffi et al., 2018). To the best of our knowledge, this is the first report on the genetic diversity data for veteran oriental plane trees within heritage sites, which will offer helpful information for their management and conservation.

Keywords: Gardens of XVI and XVII century; Genetic variation; Inter Simple Sequence Repeat; Management and conservation of ancient plant material; Platanus orientalis L.; Simple-sequence repeats; Veteran trees.