Combining Biocontrol Agent With Plant Nutrients for Integrated Control of Tomato Early Blight Through the Modulation of Physio-Chemical Attributes and Key Antioxidants

Zoia Arshad Awan; Amna Shoaib; Muhammad Sarmad Iftikhar; Basit Latief Jan; Parvaiz Ahmad

doi:10.3389/fmicb.2022.807699

Combining Biocontrol Agent With Plant Nutrients for Integrated Control of Tomato Early Blight Through the Modulation of Physio-Chemical Attributes and Key Antioxidants

Front Microbiol. 2022 Mar 23:13:807699. doi: 10.3389/fmicb.2022.807699. eCollection 2022.

Authors

Zoia Arshad Awan¹, Amna Shoaib¹, Muhammad Sarmad Iftikhar², Basit Latief Jan³, Parvaiz Ahmad⁴

Affiliations

¹ Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
² School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.
³ Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
⁴ Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia.

Abstract

Early blight (EB) is one of the major fungal diseases caused by Alternaria solani that is responsible for destructive tomato production around the globe. Biocontrol agent/s can be adequately implemented in an integrated management framework by using it in combination with vital plant nutrients, e.g., nitrogen, phosphorus, and potassium (NPK) and zinc (Zn). The current study was aimed to assess the integrated effect of a biocontrol agent Bacillus subtilis (BS-01) and the selective plant nutrients (NPK and Zn) on EB disease management and tomato crop performance. A field experiment was conducted for the off-season tomato production (under walk-in tunnels) in Punjab, Pakistan. The trial was set in a randomized complete block design (RCBD) and comprised nine treatments of a biocontrol agent (BS-01) either alone or in combination with the plant nutrients, viz., NPK (64:46:50 kg acre^-1) and Zn (10 kg acre^-1) as sustainable disease managing approach against EB. In addition, the biocontrol efficacy of B. subtilis (BS-01) on a fungal load of A. solani was estimated by quantitative PCR assays, where the foliar application of BS-01 on tomato plants either alone or in combination with the plant nutrients was done as a preventive measure. Our results revealed that the interactive effect of BS-01 with plant nutrients conferred significantly a varying degree of resilience in the infected tomato plants against EB by effectively modifying the content of total chlorophyll, carotenoids, and total phenolics along with the activities of antioxidant enzymes (SOD, CAT, POX, PPO, and PAL). In addition, the integrative effect of BS-01 and plant nutrients proved significantly effective in reducing pathogen load on inoculated tomato foliage, displaying the desired level of protection against A. solani infection. Besides, the complementary interaction of BS-01 + Zn + NPK worked synergistically to improve crop productivity by providing the highest marketable yield (21.61 tons acre^-1) and net profit (361,363 Pakistani rupees acre^-1). This integrated approach is put forward as a way to reduce the fungicide doses to control EB that would act as a sustainable plant protection strategy to generate profitable tomato production.

Keywords: biocontrol; early blight; integrated disease management; qPCR; tomato production.