A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

Abdulaziz A Al-Askar; Ehsan M Rashad; Zeiad Moussa; Khalid M Ghoneem; Ashraf A Mostafa; Fatimah O Al-Otibi; Amr Abker Arishi; WesamEldin I A Saber

doi:10.3389/fmicb.2022.772417

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

Front Microbiol. 2022 Mar 10:13:772417. doi: 10.3389/fmicb.2022.772417. eCollection 2022.

Authors

Abdulaziz A Al-Askar¹, Ehsan M Rashad², Zeiad Moussa³, Khalid M Ghoneem², Ashraf A Mostafa¹, Fatimah O Al-Otibi¹, Amr Abker Arishi⁴, WesamEldin I A Saber³

Affiliations

¹ Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
² Department of Seed Pathology Research, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
³ Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt.
⁴ School of Molecular Sciences, The University of Western Australia, Perth, WA, Australia.

Abstract

Plant residuals comprise the natural habitat of the plant pathogen; therefore, attention is currently focusing on biological-based bioprocessing of biomass residuals into benefit substances. The current study focused on the biodegradation of peanut plant residual (PNR) into citric acid (CA) through a mathematical modeling strategy. Novel endophytic Trichoderma longibrachiatum WKA55 (GenBank accession number: MZ014020.1), having lytic (cellulase, protease, and polygalacturonase) activity, and tricalcium phosphate (TCP) solubilization ability were isolated from peanut seeds and used during the fermentation process. As reported by HPLC, the maximum CA (5505.1 μg/g PNR) was obtained after 9 days in the presence of 15.49 mg TCP, and 15.68 mg glucose. GC-MS analysis showed other bioactive metabolites in the filtrate of the fermented PNR. Practically, the crude product (40%) fully inhibited (100%) the growth and spore germination of three mycotoxinogenic fungi. On peanuts, it improved the seed germination (91%), seedling features, and vigor index (70.45%) with a reduction of abnormal seedlings (9.33%). The current study presents the fundamentals for large-scale production in the industry for the sustainable development of PNR biomass as a natural source of bioactive metabolites, and safe consumption of lignocellulosic-proteinaceous biomass, as well. T. longibrachiatum WKA55 was also introduced as a novel CA producer specified on PNR. Application of the resulting metabolite is encouraged on a large scale.

Keywords: bioactive metabolites; biocontrol; biomass; citric acid; fermentation; hydrolytic enzymes; peanut; response surface methodology.