The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression

Kaja Kasarello; Iwona Köhling; Anna Kosowska; Katarzyna Pucia; Anna Lukasik; Agnieszka Cudnoch-Jedrzejewska; Leszek Paczek; Urszula Zielenkiewicz; Piotr Zielenkiewicz

doi:10.3389/fphar.2022.846830

The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression

Front Pharmacol. 2022 Mar 24:13:846830. doi: 10.3389/fphar.2022.846830. eCollection 2022.

Authors

Affiliations

¹ Centre for Preclinical Research, Department of Experimental and Clinical Physiology, Medical University of Warsaw, Warsaw, Poland.
² Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
³ Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
⁴ Department of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Warsaw, Poland.
⁵ Faculty of Biology, University of Warsaw, Warsaw, Poland.

Abstract

Recently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species ("xenomiRs"), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation via the use of cabbage leaf compresses, we investigated the effects of Brassica oleracea derived miR172a on the potential human target gene encoding FAN (Factor Associated with Neutral Sphingomyelinase Activation) protein. In vitro experiments showed a decrease in FAN protein levels in both human and mouse cells transfected with bol-miRNA172a. As the FAN protein mediates inflammatory responses, the potential of miR172a to mitigate the inflammatory process was tested in a mouse model of rheumatoid arthritis. Animal studies showed the decreased oedema of inflamed paws in mouse with rheumatoid arthritis model induced after treatment with miR172a.

Keywords: FAN protein; collagen-induced arthritis; inflammation; rheumatoid arthritis; xenomiRs.