Mapping chromatin loops in single cells

Miao Yu; Yun Li; Ming Hu

doi:10.1016/j.tig.2022.03.007

Mapping chromatin loops in single cells

Trends Genet. 2022 Jul;38(7):637-640. doi: 10.1016/j.tig.2022.03.007. Epub 2022 Apr 7.

Authors

Miao Yu¹, Yun Li², Ming Hu³

Affiliations

¹ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
² Department of Genetics, University of North Carolina, Chapel Hill, NC, USA; Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA; Department of Computer Science, University of North Carolina, Chapel Hill, NC, USA.
³ Department of Quantitative Health Sciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA. Electronic address: hum@ccf.org.

Abstract

Recent advances in high-throughput chromatin conformation capture (Hi-C) technologies at the single-cell level enable the identification of cell type-specific chromatin loops directly from complex tissues. This may help to interpret noncoding variants identified by genome-wide association studies (GWAS) in disease-relevant cell types. We briefly review current experimental and computational strategies for mapping chromatin loops in single cells.

Keywords: chromatin loops; chromatin spatial organization; single-cell Hi-C.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Chromatin* / genetics
Chromosome Mapping
Chromosomes
Genome-Wide Association Study*
Molecular Conformation

Substances

Chromatin

Abstract

Publication types

MeSH terms

Substances

Grants and funding