Structural characterization and bioactivity of novel exopolysaccharides produced by Tetragenococcus halophilus

Min Zhang; Siyuan Zeng; Liying Hao; Shangjie Yao; Dingkang Wang; Huan Yang; Chongde Wu

doi:10.1016/j.foodres.2022.111083

Structural characterization and bioactivity of novel exopolysaccharides produced by Tetragenococcus halophilus

Food Res Int. 2022 May:155:111083. doi: 10.1016/j.foodres.2022.111083. Epub 2022 Mar 1.

Authors

Min Zhang¹, Siyuan Zeng², Liying Hao³, Shangjie Yao¹, Dingkang Wang¹, Huan Yang¹, Chongde Wu⁴

Affiliations

¹ College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, China.
² College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
³ State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
⁴ College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, China. Electronic address: cdwu@scu.edu.cn.

PMID: 35400459
DOI: 10.1016/j.foodres.2022.111083

Abstract

In this study, exopolysaccharides produced by Tetragenococcus halophilus were extracted and purified. Two fractions named EPS-1 and EPS-2 were obtained with average molecular weights of 2613.4 and 93.4 kDa, respectively. Analysis of the structures demonstrated that EPS-1 was mainly composed of galactose, glucose, mannose and glucuronic acid, and EPS-2 mostly consisted of glucose and mannose. 800 MHz nuclear magnetic resonance spectroscopy elucidated different and partial primary structures of EPS-1 and EPS-2. The microtopography was observed by atomic force microscope and scanning electron microscope. In addition, the two fractions exhibited antioxidant activities on DPPH, hydroxyl and ABTS^•+ radicals. Furthermore, EPS-1 and EPS-2 could protect Lactococcus lactis against cryogenic and lyophilized stress. These results indicated that exopolysaccharides from T. halophilus in this study would have great potential in the application of food and pharmaceutical industry.

Keywords: Antioxidant activity; Bioactivity; Cytoprotection; Exopolysaccharide; Structural characterization; Tetragenococcus halophilus.

MeSH terms

Antioxidants / chemistry
Antioxidants / pharmacology
Enterococcaceae
Glucose
Mannose*
Polysaccharides, Bacterial* / chemistry

Substances

Antioxidants
Polysaccharides, Bacterial
Glucose
Mannose

Supplementary concepts

Tetragenococcus halophilus