The aim of this study was to scientifically investigate the impact of optimal-water boiling cooking on the volatile profile of 26 japonica rice varieties. A modified direct solvent extraction combined with gas chromatography-mass spectrometry was applied to analyze the volatiles in raw and cooked samples. 2-Acetyl-1-pyrroline (2AP) was only detected in aromatic varieties and decreased after cooking (-81.94% - -43.97%). Great losses of esters and long-chain ketones were exhibited after cooking. Cooking accelerated lipid oxidation and degradation of phenolic acids, thus volatiles originate from lipid oxidation and several benzenoid compounds showed increase trends in majority of the samples. Increases of 3 saturated aldehydes including hexanal (48.01% - 306.02%), octanal and nonanal (67.03% - 544.15%) could be observed in all samples. Acetophenone, 2-methoxy-4-vinylphenol (11.95% - 297.61%) and vanillin (14.29% - 319.25%) were also enhanced upon cooking. Correlations existed in volatiles with common precursors or involved in same pathways. According to multivariate and univariate statistical analysis, 18 volatiles most greatly influenced by cooking were selected. This study will facilitate the aroma improvement of cooked rice in the future.
Keywords: Cooking; OPLS-DA; PCA; Rice; Volatile.
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