Pilot study in human healthy volunteers on the mechanisms underlying remote ischemic conditioning (RIC) - Targeting circulating immune cells and immune-related proteins

Inês Mollet; Catarina Martins; Miguel Ângelo-Dias; Ana S Carvalho; Kerman Aloria; Rune Matthiesen; Miguel Viana-Baptista; Luís Miguel Borrego; Helena L A Vieira

doi:10.1016/j.jneuroim.2022.577847

Pilot study in human healthy volunteers on the mechanisms underlying remote ischemic conditioning (RIC) - Targeting circulating immune cells and immune-related proteins

J Neuroimmunol. 2022 Jun 15:367:577847. doi: 10.1016/j.jneuroim.2022.577847. Epub 2022 Mar 18.

Authors

Inês Mollet¹, Catarina Martins², Miguel Ângelo-Dias², Ana S Carvalho³, Kerman Aloria⁴, Rune Matthiesen³, Miguel Viana-Baptista⁵, Luís Miguel Borrego⁶, Helena L A Vieira⁷

Affiliations

¹ UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Caparica, Portugal; CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal.
² CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal; Comprehensive Health Research Centre (CHRC), NOVA Medical School, Universidade NOVA de Lisboa, Portugal.
³ CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal.
⁴ Proteomics Core Facility-SGIKER, University of the Basque Country UPV/EHU, Vizcaya, Spain.
⁵ CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal; Department of Neurology, Hospital de Egas Moniz, Centro Hospitalar Lisboa Ocidental, Lisboa, Portugal.
⁶ CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal; Comprehensive Health Research Centre (CHRC), NOVA Medical School, Universidade NOVA de Lisboa, Portugal; Immunoallergy Department, Hospital da Luz Lisboa, Portugal.
⁷ UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Caparica, Portugal; CEDOC - Faculdade de Ciências Médicas, NOVA Medical School, Universidade NOVA de Lisboa, Portugal; Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal. Electronic address: hl.vieira@fct.unl.pt.

PMID: 35398724
DOI: 10.1016/j.jneuroim.2022.577847

Abstract

Remote ischemic conditioning (RIC) is a novel promising therapy for treatment of neurological diseases, including ischemic stroke. RIC consists of short cycles of ischemia in a distant non-vital organ that may protect other organs against ischemia. Extensive experimental data and some few clinical trials support the neuroprotective role of RIC in ischemic stroke. Nevertheless, the circulating factors involved in this inter-organ communication and neuroprotection are not clarified. This pilot study in humans characterized the innate and adaptive circulating immune cell populations following RIC. This analysis has a particular focus at 24 h after RIC to avoid circadian influence. In silico functional analysis of mass spectrometry data identified 15 immune-related proteins. Our results reveal an immune response following RIC.

Keywords: Adaptive response; Innate response; Neuroprotection; Remote ischemic conditioning; Stroke.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Healthy Volunteers
Humans
Ischemia
Ischemic Preconditioning* / methods
Ischemic Stroke*
Pilot Projects