Simultaneous analysis of free phytosterols and phytosterol glycosides in rice bran by SPE/GC-MS

Simin Feng; Liling Wang; Ping Shao; Baiyi Lu; Yanfei Chen; Peilong Sun

doi:10.1016/j.foodchem.2022.132742

Simultaneous analysis of free phytosterols and phytosterol glycosides in rice bran by SPE/GC-MS

Food Chem. 2022 Sep 1:387:132742. doi: 10.1016/j.foodchem.2022.132742. Epub 2022 Mar 19.

Authors

Simin Feng¹, Liling Wang², Ping Shao³, Baiyi Lu⁴, Yanfei Chen⁵, Peilong Sun⁶

Affiliations

¹ Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China. Electronic address: fengsimin@zjut.edu.cn.
² Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China.
³ Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China.
⁴ College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China.
⁵ Zhejiang Delekang Food Co., Ltd., Taizhou 318000, Zhejiang, People's Republic of China.
⁶ Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China. Electronic address: sun_pl@zjut.edu.cn.

PMID: 35398677
DOI: 10.1016/j.foodchem.2022.132742

Abstract

In this study, free phytosterols and phytosterol glycosides in rice bran were successfully separated and analyzed using solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that free phytosterols in rice bran included ergosterol (129 ± 8 μg/g rice bran), campesterol (126 ± 9 μg/g rice bran), stigmasterol (106 ± 9 μg/g rice bran), β-sitosterol (305 ± 10 μg/g rice bran), cycloartenol (80.5 ± 3.9 μg/g rice bran) and 24-methylenecycloartenol (87.1 ± 2.2 μg/g rice bran), while phytosterol glycosides included campesterol glucoside (16.0 ± 1.3 μg/g rice bran), stigmasterol glucoside (99.0 ± 4.9 μg/g rice bran) and β-sitosterol glucoside (133 ± 7 μg/g rice bran). The methodological validation indicated that this method could accurately quantify free phytosterols and phytosterol glycosides in rice bran. This study provided a new direction to establish a rapid and simple method for the simultaneous determination of different forms of phytosterols in foods.

Keywords: Free phytosterols; Gas chromatography–mass spectrometry; Methodological validation; Phytosterol glycosides; Rice bran; Solid-phase extraction.

MeSH terms

Gas Chromatography-Mass Spectrometry
Glycosides
Oryza* / chemistry
Phytosterols* / analysis
Solid Phase Extraction

Substances

Glycosides
Phytosterols