Mechanistic Insights into the Multiple Activities of the Rad5 Family of Enzymes

Miaomiao Shen; Wei Huang; Fangjie Qiu; Hengyao Niu; Song Xiang

doi:10.1016/j.jmb.2022.167581

Mechanistic Insights into the Multiple Activities of the Rad5 Family of Enzymes

J Mol Biol. 2022 May 30;434(10):167581. doi: 10.1016/j.jmb.2022.167581. Epub 2022 Apr 7.

Authors

Miaomiao Shen¹, Wei Huang², Fangjie Qiu², Hengyao Niu³, Song Xiang⁴

Affiliations

¹ Department of Biochemistry and Molecular Biology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, 300070 Tianjin, PR China; Tianjin Medical University Cancer Institute and Hospital, 300060 Tianjin, PR China.
² Department of Biochemistry and Molecular Biology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, 300070 Tianjin, PR China.
³ Department of Molecular and Cellular Biochemistry, Indiana University Bloomington, 47405 IN, USA.
⁴ Department of Biochemistry and Molecular Biology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, 300070 Tianjin, PR China. Electronic address: xiangsong@tmu.edu.cn.

PMID: 35398319
DOI: 10.1016/j.jmb.2022.167581

Abstract

The budding yeast protein Rad5 is highly conserved among eukaryotes. Rad5 and its orthologs including helicase like transcription factor (HLTF) and SNF2 histone linker PHD RING helicase (SHPRH) in humans constitute a unique family of enzymes that play critical roles in the cellular response to DNA replication stresses. The function of the Rad5 family of enzymes is fulfilled by their multiple activities, including ubiquitin ligase, replication fork regression activities and others. Herein, we review recent studies that provided mechanistic insights into their multiple activities and their coordination.

Keywords: DNA damage tolerance; fork regression; protein structure; replication stress response; ubiquitin ligase.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / metabolism
DNA Damage
DNA Helicases* / classification
DNA Helicases* / genetics
DNA Helicases* / metabolism
DNA Replication*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Humans
Saccharomyces cerevisiae Proteins* / classification
Saccharomyces cerevisiae Proteins* / genetics
Saccharomyces cerevisiae Proteins* / metabolism
Transcription Factors / genetics
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism

Substances

DNA-Binding Proteins
HLTF protein, human
Saccharomyces cerevisiae Proteins
Transcription Factors
SHPRH protein, human
Ubiquitin-Protein Ligases
Adenosine Triphosphatases
RAD5 protein, S cerevisiae
DNA Helicases