Selective and sensitive detection and detoxification of Pd2+ in living cells with a water-soluble fluorescent probe

Lidan Zhang; Chuang Wang; Yunhan Jiang; Huidong Li; Huizhen Wang; Haiyue Long; Pingxian Liu; Xiaoqiu Xu; Tao Yang

doi:10.1016/j.aca.2022.339728

Selective and sensitive detection and detoxification of Pd²⁺ in living cells with a water-soluble fluorescent probe

Anal Chim Acta. 2022 Apr 29:1204:339728. doi: 10.1016/j.aca.2022.339728. Epub 2022 Mar 22.

Authors

Lidan Zhang¹, Chuang Wang¹, Yunhan Jiang², Huidong Li³, Huizhen Wang³, Haiyue Long⁴, Pingxian Liu¹, Xiaoqiu Xu¹, Tao Yang⁵

Affiliations

¹ Laboratory of Human Diseases and Immunotherapy, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Institute of Immunology and Inflammation, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
² Laboratory of Human Diseases and Immunotherapy, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, China; Institute of Immunology and Inflammation, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
³ School of Science, Key Laboratory of High-Performance Scientific Computation, Xihua University, Chengdu, 610041, China.
⁴ Department of Pharmacy, The Air Force Hospital of Western Theater Command, Chengdu, 610041, China.
⁵ Laboratory of Human Diseases and Immunotherapy, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Institute of Immunology and Inflammation, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China. Electronic address: yangtao@wchscu.cn.

PMID: 35397916
DOI: 10.1016/j.aca.2022.339728

Abstract

Due to the cytotoxic potential of even low doses of Pd²⁺, the development of its detection and detoxification strategies is highly demanding. In this paper, we developed a water-soluble fluorescent probe IMQU-8 with a new scaffold for Pd²⁺ sensing. IMQU-8 can detect Pd²⁺ with high selectivity and sensitivity and has a good detection limit of 2.5 nM under physiological conditions. Its sensing mechanism has been revealed through job plot experiments and HRMS, FT-IR, ¹H NMR and DFT calculations, which demonstrated that the N atoms of the pyridyl group and imino group are crucial for Pd²⁺ sensing. Fluorescence lifetime assessment indicated that IMQU-8 and IMQU-8-Pd have almost identical fluorescence lifetimes, implying that IMQU-8 undergoes static quenching toward Pd²⁺. Additionally, IMQU-8 has been successfully applied to image Pd²⁺ in living cells. Since IMQU-8 and its coordinated complex IMQU-8-Pd exhibit low toxicity, IMQU-8 has been applied for the detoxification of Pd²⁺ in living cells.

Keywords: Cell imaging; Detoxification; Fluorescent probe; Pd(2+) sensing; Water soluble.

MeSH terms

Density Functional Theory
Fluorescent Dyes* / chemistry
Fluorescent Dyes* / toxicity
HeLa Cells
Humans
Spectroscopy, Fourier Transform Infrared
Water* / chemistry

Substances

Fluorescent Dyes
Water