Objective: To investigate the effect of acute myeloid leukemia cells in leukemia-microenvironment on proliferation and apoptosis of bone marrow-derived mesenchymal stromal cells (BM-MSC).
Methods: Acute myeloid leukemia (AML) murine models overexpressing MLL-AF9 were established. The number of BM-MSC of wild type (WT) and AML-derived mice were analyzed by flow cytometry. Morphology and growth differences between WT and AML-derived BM-MSC were analyzed by inverted fluorescence microscope. Proliferation and apoptosis of BM-MSC between these two groups were detected by Brdu and Annexin V/PI.
Results: Compared with WT-derived BM-MSC, the number and proliferation rate of AML-derived BM-MSC significantly increased (P<0.01, P<0.001), while apoptosis rate decreased (P<0.05). When cultured in vitro, BM-MSC grew faster under conditional medium.
Conclusion: AML cells can promote proliferation and inhibit apoptosis of BM-MSC.
题目: 急性髓系白血病细胞对骨髓基质细胞增殖、凋亡的影响.
目的: 研究在白血病微环境下，急性髓系白血病细胞对骨髓基质细胞（BM-MSC）增殖、凋亡的影响.
方法: 选取过表达MLL-AF9的急性髓系白血病（AML）小鼠模型与野生型（WT）小鼠为研究对象。应用流式细胞分选仪分选WT小鼠与AML小鼠来源的BM-MSC并计数。通过倒置荧光显微镜分析不同来源的BM-MSC的形态及生长差异性。采用Brdu法检测不同来源的BM-MSC增殖率。采用Annexin V/PI 法检测不同来源的BM-MSC凋亡率.
结果: 与WT小鼠来源的BM-MSC比较，AML来源的BM-MSC数量显著增加（P<0.01），细胞增殖率显著增高（P<0.001）；凋亡率显著降低（P<0.05）。在体外培养BM-MSC，加入条件培养基的BM-MSC生长速度显著加快，但形态没有明显差异.
结论: 在白血病微环境中，AML细胞能够促进BM-MSC增殖，抑制其凋亡.
Keywords: acute myeloid leukemia; apoptosis; bone marrow-derived mesenchymal stromal cell; mouse; proliferation.