Objective: To investigate the expression and correlation of miR-211, miR-155, and C-myc in acute T lymphocytic leukemia (T-ALL), aiming to provide evidence for the diagnosis and treatment.
Methods: A total of 96 T-ALL patients who were diagnosed and treated in People's Hospital of Zhengzhou from June 2014 to June 2017 were selected, and 69 healthy volunteers who had a physical examination were selected as control group in the same period. Real-time fluorescent quantitative PCR (RT-PCR) was used to determine the expression levels of miR-211, miR-155, and C-myc in peripheral blood mononuclear cells in each group. Kaplan-Meier was used to analyze the survival of T-ALL patients and correlation of miR-211, miR-155, and C-myc with prognosis. Pearson correlation analysis was used to evaluate the correlation of miR-211, miR-155, and C-myc with disease risk.
Results: The expression levels of miR-211 mRNA, miR-155 mRNA, and C-myc mRNA in T-ALL group were higher than those in the control group (P<0.01), those in non-remission group were higher than those in remission group (P<0.01), and those in high-risk group were also higher than those in low-risk group and intermediate-risk group (P<0.01). The survival time of T-ALL patients with low miR-211 expression was longer than that with high miR-211 expression (P<0.01), that with low miR-155 expression was longer than that with high miR-155 expression (P<0.01), and that with low C-myc expression was also longer than that with high C-myc expression (P<0.01). The high expression of miR-211, miR-155, and C-myc was linearly positively correlated with high risk of disease (r=0.749, 0.781, 0.804).
Conclusion: The expressions of miR-211, miR-155, and C-myc are up-regulated in T-ALL patients, closely related to prognosis, and linearly positively correlated with disease risk.
题目: 急性T淋巴细胞白血病的miR-211、miR-155、C-myc表达水平及相关性研究.
目的: 分析并探讨急性T淋巴细胞白血病（T-ALL）的miR-211、miR-155、C-myc表达及相关性，旨在为早期诊断及预后监测提供依据.
方法: 选择2014年6月-2017年6月于郑州人民医院接受诊治的T-ALL患者96例，另选同期体检健康的志愿者69例为对照组，采用实时荧光定量PCR测定各组外周血单个核细胞中miR-211、miR-155、C-myc表达水平，通过Kaplan-Meier分析T-ALL患者生存情况及miR-211、miR-155和C-myc与预后的关系，Pearson相关分析评价miR-211、miR-155和C-myc与疾病危险度的相关性.
结果: T-ALL组miR-211 mRNA、miR-155 mRNA、C-myc mRNA表达水平均明显高于对照组（P<0.01），未缓解组均明显高于缓解组（P<0.01），高危组亦明显高于低危组和中危组（P<0.01）。miR-211低表达患者生存时间明显长于高表达患者（P<0.01），miR-155低表达患者生存时间明显长于高表达患者（P<0.01），C-myc低表达患者生存时间亦明显长于高表达患者（P<0.01）。miR-211、miR-155和C-myc高表达与疾病高危呈线性正相关(r=0.749、0.781、0.804).
结论: T-ALL患者miR-211、miR-155、C-myc表达上调，其表达水平与预后密切相关，且与疾病危险度呈线性正相关.
Keywords: C-myc; acute T lymphocytic leukemia; correlation; miR-155; miR-211.