Enzymatic membrane reactor in xylose bioconversion with simultaneous cofactor regeneration

Karolina Bachosz; Adam Piasecki; Agata Zdarta; Ewa Kaczorek; Manuel Pinelo; Jakub Zdarta; Teofil Jesionowski

doi:10.1016/j.bioorg.2022.105781

Enzymatic membrane reactor in xylose bioconversion with simultaneous cofactor regeneration

Bioorg Chem. 2022 Jun:123:105781. doi: 10.1016/j.bioorg.2022.105781. Epub 2022 Apr 1.

Authors

Karolina Bachosz¹, Adam Piasecki², Agata Zdarta³, Ewa Kaczorek⁴, Manuel Pinelo⁵, Jakub Zdarta⁶, Teofil Jesionowski⁷

Affiliations

¹ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: karolina.h.bachosz@doctorate.put.poznan.pl.
² Institute of Materials Science and Engineering, Faculty of Mechanical Engineering and Management, Poznan University of Technology, Jana Pawla II 24, PL-60965 Poznan, Poland. Electronic address: adam.piasecki@put.poznan.pl.
³ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: agata.zdarta@put.poznan.pl.
⁴ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: ewa.kaczorek@put.poznan.pl.
⁵ Department of Chemical and Biochemical Engineering, Technical University of Denmark, Soltofts Plads, Building 227, DK-2800 Kongens Lyngby, Denmark. Electronic address: mp@kt.dtu.dk.
⁶ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: jakub.zdarta@put.poznan.pl.
⁷ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: teofil.jesionowski@put.poznan.pl.

PMID: 35395447
DOI: 10.1016/j.bioorg.2022.105781

Abstract

In this study, we present the concept of co-immobilization of xylose dehydrogenase and alcohol dehydrogenase from Saccharomyces cerevisiae on an XN45 nanofiltration membrane for application in the process of xylose bioconversion to xylonic acid with simultaneous cofactor regeneration and membrane separation of reaction products. During the research, the effectiveness of the co-immobilization of enzymes was confirmed, and changes in the properties of the membrane after the processes were determined. Using the obtained biocatalytic system it was possible to obtain 99% xylonic acid production efficiency under optimal conditions, which were 5 mM xylose, 5 mM formaldehyde, ratio of NAD+:NADH 1:1, and 60 min of reaction. Additionally, the co-immobilization of enzymes made it possible to improve stability of the co-immobilized enzymes and to carry out xylose conversion in six consecutive cycles and after 7 days of storage at 4 °C with over 90% efficiency. The presented data confirm the effectiveness of the co-immobilization, improvement of the stability and reusability of the biocatalysts, and show that the obtained enzymatic system is promising for use in xylose bioconversion and simultaneous regeneration of nicotinamide cofactor.

Keywords: Biomass conversion; Cofactor regeneration system; Enzyme immobilization; Xylonic acid; Xylose dehydrogenase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase*
Aldehyde Reductase
Biocatalysis
Regeneration
Xylose*

Substances

Xylose
Alcohol Dehydrogenase
Aldehyde Reductase