Cpmer: A new conserved eEF1A2-binding partner that regulates Eomes translation and cardiomyocyte differentiation

Yao Lyu; Wenwen Jia; Yukang Wu; Xin Zhao; Yuchen Xia; Xudong Guo; Jiuhong Kang

doi:10.1016/j.stemcr.2022.03.006

Cpmer: A new conserved eEF1A2-binding partner that regulates Eomes translation and cardiomyocyte differentiation

Stem Cell Reports. 2022 May 10;17(5):1154-1169. doi: 10.1016/j.stemcr.2022.03.006. Epub 2022 Apr 7.

Authors

Yao Lyu¹, Wenwen Jia¹, Yukang Wu¹, Xin Zhao¹, Yuchen Xia¹, Xudong Guo², Jiuhong Kang³

Affiliations

¹ Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, National Stem Cell Translational Resource Center, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
² Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, National Stem Cell Translational Resource Center, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; Institute for Advanced Study, Tongji University, Shanghai 200092, China. Electronic address: 19504@tongji.edu.cn.
³ Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, National Stem Cell Translational Resource Center, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address: jhkang@tongji.edu.cn.

Abstract

Previous studies have shown that eukaryotic elongation factor 1A2 (eEF1A2) serves as an essential heart-specific translation elongation element and that its mutation or knockout delays heart development and causes congenital heart disease and death among species. However, the function and regulatory mechanisms of eEF1A2 in mammalian heart development remain largely unknown. Here we identified the long noncoding RNA (lncRNA) Cpmer (cytoplasmic mesoderm regulator), which interacted with eEF1A2 to co-regulate differentiation of mouse and human embryonic stem cell-derived cardiomyocytes. Mechanistically, Cpmer specifically recognized Eomes mRNA by RNA-RNA pairing and facilitated binding of eEF1A2 with Eomes mRNA, guaranteeing Eomes mRNA translation and cardiomyocyte differentiation. Our data reveal a novel functionally conserved lncRNA that can specifically regulate Eomes translation and cardiomyocyte differentiation, which broadens our understanding of the mechanism of lncRNA involvement in the subtle translational regulation of eEF1A2 during mammalian heart development.

Keywords: Eomes; cardiomyocyte differentiation; eEF1A2; lncRNA; mRNA translation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / genetics
Eukaryota / genetics
Eukaryota / metabolism
Mammals / genetics
Mammals / metabolism
Myocytes, Cardiac* / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / metabolism

Substances

RNA, Long Noncoding
RNA, Messenger