The Inhibitory Effect and Mechanism of Ferula akitschkensis Volatile Oil on Gastric Cancer

Rong Han; Yun Sun; Ruoting Ma; Dexi Wang; Jianan Sun; Shengjun Zhao; Haiying Zhang

doi:10.1155/2022/5092742

The Inhibitory Effect and Mechanism of Ferula akitschkensis Volatile Oil on Gastric Cancer

Evid Based Complement Alternat Med. 2022 Mar 29:2022:5092742. doi: 10.1155/2022/5092742. eCollection 2022.

Authors

Rong Han^{1

2}, Yun Sun³, Ruoting Ma⁴, Dexi Wang⁴, Jianan Sun^{4

5}, Shengjun Zhao^{1

2}, Haiying Zhang^{1

2}

Affiliations

¹ Department of Pharmacy, Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University, Urumqi 830000, Xinjiang, China.
² Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine, Urumqi 830000, Xinjiang, China.
³ Department of Traditional Chinese Medicine, The Traditional Chinese Medicine College of Xinjiang Medical University, Urumqi 83000, Xinjiang, China.
⁴ Graduate School, The Fourth Clinical College of Xinjiang Medical University, Urumqi 83000, Xinjiang, China.
⁵ State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi 830000, Xinjiang, China.

Abstract

Ferula akitschkensis volatile oil (FAVO) has a good inhibitory activity on gastric cancer cell proliferation, but the mechanism of action is not yet clear. In this study, we tested the antigastric cancer efficacy and mechanism of FAVO using both in vivo and in vitro models. The results showed that FAVO effectively inhibited the proliferation, migration, and invasion of human gastric cancer SGC-7901 cells, the formation of small tubules of human umbilical vein endothelial cells as well as zebrafish intersegmental vessel and intestinal vein angiogenesis. In vivo experiments showed that FAVO significantly delayed the growth of SGC-7901 tumor-bearing nude mice and induced higher serum IL-2 and IFN-γ and reduced serum IL-6. Western blot results showed that FAVO reduced the expression of HIF-2α, VEGF, VEGFR2, P-VEGFR2, Akt, and P-Akt in SGC-7901 cells with CoCl₂ induced hypoxia. We further clarified the main chemical components of FAVO through GC-MS analysis. In summary, FAVO may inhibit tumor growth and angiogenesis via inhibiting the HIF-2α/VEGF signaling pathway.