Lymphatic Absorption of Microbial Plasmalogens in Rats

Nana Sato; Aki Kanehama; Akiko Kashiwagi; Miwa Yamada; Megumi Nishimukai

doi:10.3389/fcell.2022.836186

Lymphatic Absorption of Microbial Plasmalogens in Rats

Front Cell Dev Biol. 2022 Mar 22:10:836186. doi: 10.3389/fcell.2022.836186. eCollection 2022.

Authors

Nana Sato¹, Aki Kanehama¹, Akiko Kashiwagi², Miwa Yamada^{1

3}, Megumi Nishimukai^{3

4}

Affiliations

¹ Faculty of Agriculture, Department of Biological Chemistry and Food Science, Iwate University, Morioka, Japan.
² Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan.
³ Agri-Innovation Center, Iwate University, Morioka, Japan.
⁴ Department of Animal Science, Faculty of Agriculture, Iwate University, Morioka, Japan.

Abstract

Plasmalogens, functional glycerophospholipids with biological roles in the human body, are associated with various diseases. Although a variety of saturated and/or unsaturated fatty acids in plasmalogens are presumed to have different functions in the human body, there are limited reports validating such functions of plasmalogens. In this study, we focused on the bacterial plasmalogen derived from Selenomonas ruminantium subsp. lactilytica (NBRC No. 103574) with different main species of hydrocarbon chains at the sn-1 position and shorter fatty acids at the sn-2 position than animal plasmalogens. Optimum culture conditions of S. ruminantium for high-yield production of plasmalogens, such as pH and the concentration of caproic acid, were investigated under anaerobic conditions using a 2-L scale jar fermenter. The obtained plasmalogen mainly consisted of the ethanolamine plasmalogen (PlsEtn). The molar ratios of PlsEtn species obtained from S. ruminantium, at sn-1/sn-2 positions, were p16:1/14:0 (68.4%), p16:1/16:1 (29.2%), p16:1/16:0 (0.7%), p16:1/15:0 (0.3%), and p17:1/14:0 (0.3%). Subsequently, duodenal infusion of the emulsion carrying the lipid extracted from S. ruminantium was carried out in lymph duct-cannulated rats. In the lymphatic plasmalogen of rats, the level of PlsEtns with molar ratios p16:1/14:0 and p16:1/16:1, the main species of plasmalogens from S. ruminantium, increased gradually until 3-4 h after lipid injection and then gradually decreased. In addition, the level of PlsEtns with p16:1/20:4 and p16:1/22:6 rapidly increased, peaking at 1-1.5 h and 1.5-2 h after lipid injection, respectively. The increase in the number of PlsEtns with p16:1/20:4 and p16:1/22:6 suggested that 20:4 and 22:6, the main fatty acids at the sn-2 position in the rat lymphatic plasmalogen, were preferentially re-esterified at the sn-2 position, regardless of the types of hydrocarbon chains at the sn-1 position. Thus, we showed that bacterial PlsEtns with "unnatural" structures against rats could be absorbed into the lymph. Our findings provide insights into the association between the chemical structure of plasmalogens and their biological functions in humans.

Keywords: Selenomonas ruminantium; ethanolamine plasmalogen; lymphatic absorption; microbial plasmalogen; molecular species.