The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Hui-Ning Liu; Wei-Hua Dong; Yan Lin; Zhao-Hui Zhang; Tian-Yun Wang

doi:10.3389/fbioe.2022.832065

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Front Bioeng Biotechnol. 2022 Mar 21:10:832065. doi: 10.3389/fbioe.2022.832065. eCollection 2022.

Authors

Hui-Ning Liu^{1

2}, Wei-Hua Dong^{2

3}, Yan Lin², Zhao-Hui Zhang¹, Tian-Yun Wang^{2

3}

Affiliations

¹ The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
² International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang, China.
³ Department of Biochemistry and Molecular Biology, Basic Medical School, Xinxiang Medical University, Xinxiang, China.

Abstract

Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity ensuring the good quality of RTPs produced by CHO cells. MicroRNAs(miRNAs) are short regulatory non-coding RNAs that can regulate cellular behavior and complex phenotypes. It has been found that miRNAs can enhance the expression level of recombinant proteins in CHO cells by promoting proliferation, resisting apoptosis, and regulating metabolism. miRNAs also can affect the quality of RTPs. In this review, we will discuss the effect and mechanism of miRNA on the expression level and quality of recombinant proteins in CHO cells.

Keywords: CHO cells; cell engineering; miRNA; protein quality; recombinant protein.

Publication types

Review