Characterization of the complete chloroplast genome sequence of Chinese endemic species of Nouelia insignis (Hyalideae, Asteraceae) and its phylogenetic implications

Xiaofeng Liu; Maoyun Han; Jia Chen; Xun Zhang; Zhiyu Chen; Yi Tang; Tianmeng Qu; Chunping Huang; Shuhua Yu; Zhixi Fu

doi:10.1080/23802359.2021.1921629

Characterization of the complete chloroplast genome sequence of Chinese endemic species of Nouelia insignis (Hyalideae, Asteraceae) and its phylogenetic implications

Mitochondrial DNA B Resour. 2022 Apr 1;7(4):600-602. doi: 10.1080/23802359.2021.1921629. eCollection 2022.

Authors

Xiaofeng Liu¹, Maoyun Han², Jia Chen³, Xun Zhang⁴, Zhiyu Chen¹, Yi Tang¹, Tianmeng Qu¹, Chunping Huang^{1

5}, Shuhua Yu^{1

5}, Zhixi Fu^{1

5

6}

Affiliations

¹ College of Life Sciences, Sichuan Normal University, Chengdu, China.
² Natural Resources Bureau of Maoxian, Maoxian, China.
³ Chengdu Foreign Languages School, Chengdu, China.
⁴ Jinjiang Experimental School, Chengdu, China.
⁵ Institute of Application and Development of Plant Resources, Sichuan Normal University, Chengdu, China.
⁶ Sustainable Development Research Center of Resources and Environment of Western Sichuan, Sichuan Normal University, Chengdu, China.

Abstract

This study was the first report complete chloroplast genome of Nouelia insignis (Asteraceae, Hyalideae), the large shrubs to small trees endemic to China. The circular whole cp genome of N. insignis was 151,524 bp in length, containing a large single-copy (LSC) region of 83,145 bp and a small single-copy (SSC) region of 18,261 bp. These two regions were separated by a pair of inverted repeat regions (IRa and IRb), each of them 25,060 bp in length. A total of 135 functional genes were encoded, consisting of 89 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The overall GC content of the chloroplast genome sequence was 37.8%, and the GC contents of the LSC, SSC, and IR regions were 35.9, 31.5, and 43.2%, respectively. The phylogenetic analysis by the Bayesian analysis showed that the species of N. insignis was sister group with Gerbera jamesonii by strong support values, and thus was closely related to members of subfamilies of Cichorioideae and Pertyoideae. These results will be useful for the future studies of Asteraceae in the worldwide.

Keywords: Nouelia insignis; complete chloroplast genome; phylogenomics analysis.

Grants and funding

This work was supported by the National Natural Science Foundation of China under Grant [32000158], The Special Research Project of National Tradition Chinese Medicine Industry, the Fourth National Survey on Chinese Material Medica Resources under Grant [GZY-KJS-2018-004]; The Sichuan Science and Technology Research Projects of Traditional Chinese Medicine under Grant [2018PC005]; the Project of Sustainable Development Research Center of Resources and Environment of Western Sichuan under Grant [2020CXZYHJZX03]; the Philosophy and Social Science Key Research Base Project of Sichuan Province, Sichuan Nationalities and Mountain Economy Development Research Center under Grant [SDJJ1907]; The Research and Demonstration of Fast Restoration Model of Forest and Grass Vegetation Program in the Damaged Habitat of Rare Animals of Jiuzhaigou Earthquake Stricken Area [2018-128].