The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

Jing Jing Wang; Ying Liu; Zhou Ding; Le Zhang; Caiqin Han; Changchun Yan; Eric Amador; Liqin Yuan; Ying Wu; Chunyuan Song; Ying Liu; Wei Chen

doi:10.1016/j.bioactmat.2021.12.036

The exploration of quantum dot-molecular beacon based MoS₂ fluorescence probing for myeloma-related Mirnas detection

Bioact Mater. 2022 Jan 7:17:360-368. doi: 10.1016/j.bioactmat.2021.12.036. eCollection 2022 Nov.

Authors

Jing Jing Wang¹, Ying Liu¹, Zhou Ding¹, Le Zhang¹, Caiqin Han¹, Changchun Yan¹, Eric Amador², Liqin Yuan³, Ying Wu¹, Chunyuan Song⁴, Ying Liu¹, Wei Chen^{2

5}

Affiliations

¹ Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China.
² Department of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USA.
³ Department of General Surgery, The Second Xiangya Hospital, Central South University, ChangSha, Hu'nan, 410011, China.
⁴ Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, China.
⁵ Medical Technology Research Centre, Chelmsford Campus, Anglia Ruskin University, Chelmsford, CM1 1SQ, UK.

Abstract

Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS₂ (QD-MB @MoS₂) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

Keywords: Duplex-specific nuclease (DSN); MoS2; Molecular beacon; Multiple myeloma; Quantum dot; miRNA-150; miRNA-155.