Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells

Yibin Zhang; Dilka Liyana Arachchige; Adenike Olowolagba; Rudy L Luck; Haiying Liu

doi:10.1016/j.ymeth.2022.03.019

Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells

Methods. 2022 Aug:204:22-28. doi: 10.1016/j.ymeth.2022.03.019. Epub 2022 Apr 2.

Authors

Yibin Zhang¹, Dilka Liyana Arachchige², Adenike Olowolagba², Rudy L Luck³, Haiying Liu⁴

Affiliations

¹ Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: yibinz@mtu.edu.
² Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA.
³ Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: rluck@mtu.edu.
⁴ Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: hyliu@mtu.edu.

Abstract

A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.

Keywords: Cellular Imaging; Computation chemistry; NADH; Near-infrared fluorescent probe.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Fluorescence
Fluorescent Dyes*
HeLa Cells
Humans
NAD*
Rhodamines

Substances

Fluorescent Dyes
Rhodamines
NAD

Grants and funding

R15 GM114751/GM/NIGMS NIH HHS/United States