Toxicological effects of WS2 nanomaterials on rice plants and associated soil microbes

Abstract

As an important member of transition-metal dichalcogenides family, tungsten disulfides nanomaterials (WS₂ NMs) have a wide range of applications. To date, their environmental risks remain largely unknown. In this study, rice plants were grown in soil amended with different concentrations (0, 10, and 100 mg/kg) of WS₂ NMs for 4 weeks. WS₂ NMs at 100 mg/kg significantly increased MDA (malondialdehyde) content and decreased total antioxidant capacities of leaves, indicating the oxidative response induced by WS₂ NMs. Meanwhile, WS₂ NMs at 100 mg/kg significantly decreased root biomass compared to control, indicating the negative impacts of WS₂ NMs on plant growth. While exposure to 100 mg/kg WS₂ NMs significantly increased soil bioavailable Cu, Fe, Zn, and Olsen-P, and increased the content of Cu, Fe, Zn, and P in rice leaves. Inductively coupled plasma-optical emission spectroscopy (ICP-OES) analysis showed that W was taken up by rice roots and translocated into leaves. The impact of WS₂ on soil microbial communities was evaluated by 16S rRNA gene sequencing. WS₂ NMs at 100 mg/kg significantly decreased soil microbial diversity, as indicated by decreased Shannon index. In addition, 100 mg/kg WS₂ shifted the soil microbial profile, the relative abundance of the phylum Acidobacteriota decreased, and Actinobacteriota increased. Taken together, the soil microbial community's diversity and composition have been altered upon exposure to 100 mg/kg WS₂ NMs. The results of this study provide some basic information regarding the environmental behavior and phytotoxicity of WS₂ NMs, which is valuable for safe use of WS₂ NMs.

Keywords: Rice; Soil microbes; Toxicological; Tungsten disulfide.