There are several commercial chromatographic systems for protein purification; however, development of cost-effective 3H-grade (high yield, high purity, and high activity) purification approaches is highly demanded. Here, we establish a methodology for encapsulating the IM7-displaying yeast cells in calcium alginate beads. Taking advantage of this biomaterial-based affinity chromatography, rapid and cost-effective purification of proteins with over 90% purity in a single step is achieved. Moreover, our system enables coating the multienzyme complex to produce reusable immobilized cells for efficient cascade biotransformation. Together, the present method has great application potentials not only in the laboratory but also in the industry for production of protein products as well as biocatalysis.
Keywords: Colicin E7/IM7 affinity chromatography; biocatalysis; enzyme immobilization; multiple-enzyme complex; one-step protein purification.
Copyright © 2022 Yin, Wang, Liao, Ma, Qiao, Liu, Song and Liu.