MicroED: conception, practice and future opportunities

Max T B Clabbers; Anna Shiriaeva; Tamir Gonen

doi:10.1107/S2052252521013063

MicroED: conception, practice and future opportunities

IUCrJ. 2022 Jan 18;9(Pt 2):169-179. doi: 10.1107/S2052252521013063. eCollection 2022 Mar 1.

Authors

Max T B Clabbers¹, Anna Shiriaeva¹, Tamir Gonen^{1

2

3}

Affiliations

¹ Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
² Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.
³ Department of Physiology, University of California, Los Angeles, CA 90095, USA.

Abstract

This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today's application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.

Keywords: MicroED; cryo-EM; crystallography; membrane proteins; microcrystal electron diffraction.

Publication types

Review

Grants and funding

P41 GM136508/GM/NIGMS NIH HHS/United States