From Fish Scale Gelatin to Tyrosinase Inhibitor: A Novel Peptides Screening Approach Application

Zi-Zi Hu; Xiao-Mei Sha; Lu Zhang; Min-Jun Zha; Zong-Cai Tu

doi:10.3389/fnut.2022.853442

From Fish Scale Gelatin to Tyrosinase Inhibitor: A Novel Peptides Screening Approach Application

Front Nutr. 2022 Mar 18:9:853442. doi: 10.3389/fnut.2022.853442. eCollection 2022.

Authors

Zi-Zi Hu¹, Xiao-Mei Sha¹, Lu Zhang¹, Min-Jun Zha¹, Zong-Cai Tu^{1

2}

Affiliations

¹ National R&D Center for Freshwater Fish Processing, College of Chemistry and Chemical Engineering & College of Life Science, Jiangxi Normal University, Nanchang, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.

Abstract

Bioaffinity ultrafiltration combined with LC-Orbitrap-MS/MS was applied for the first time to achieve rapid screening and identification of tyrosinase inhibitory peptides (TYIPs) from grass carp scale gelatin hydrolysates. The binding mode of TYIPs with tyrosinase was investigated by molecular docking technology. The whitening effect of TYIPs was further studied by evaluating the tyrosinase activity and melanin content in mouse B16F10 cells. Four new TYIPs were screened from hydrolysates, among which DLGFLARGF showed the strongest tyrosinase inhibition with an IC₅₀ value of 3.09 mM. Molecular docking showed that hydrogen bonds were the main driving force in the interaction between the peptide DLGFLARGF and tyrosinase. The addition of DLGFLARGF significantly inhibited the tyrosinase activity and melanin production of B16F10 melanoma cells. These results suggest that DLGFLARGF is a promising skin whitening agent for the treatment of potential pigment-related diseases.

Keywords: LC-Orbitrap-MS/MS; bioaffinity ultrafiltration; grass carp scale gelatin; tyrosinase inhibitory peptides; whitening.