LINC01087 Promotes the Proliferation, Migration, and Invasion of Thyroid Cancer Cells by Upregulating PPM1E

Ying Yin; Jianhao Huang; Hongyan Shi; Yijie Huang; Ziyang Huang; Muye Song; Liping Yin

doi:10.1155/2022/7787378

LINC01087 Promotes the Proliferation, Migration, and Invasion of Thyroid Cancer Cells by Upregulating PPM1E

J Oncol. 2022 Mar 25:2022:7787378. doi: 10.1155/2022/7787378. eCollection 2022.

Authors

Ying Yin^#¹, Jianhao Huang^#^{1

2}, Hongyan Shi^{1

3}, Yijie Huang¹, Ziyang Huang^{1

2}, Muye Song^{1

4}, Liping Yin⁵

Affiliations

¹ Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.
² Shantou University Medical College, Shantou, Guangdong, China.
³ The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China.
⁴ School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.
⁵ Imaging Department, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.

^# Contributed equally.

Abstract

This study is aimed at investigating the effect and mechanism of LINC01087 on the malignant evolution of thyroid cancer cells. The expression levels of LINC01087, miR-135a-5p, and PPM1E in thyroid carcinoma tissues were detected by QRT-PCR. Cell viability was detected using the CCK-8 method. Transwell assay was used to assess the ability of cells to invade. The targeting relationship between LINC01087 and miR-135a-5p was detected by dual luciferase reporting assay. In comparison with normal thyroid tissues and cells, the expression level of LINC01087 in thyroid cancer tissues and TPC-1 and K1 cells increased, and the expression level of miR-135a-5p in thyroid cancer tissues and TPC-1 and K1 cells decreased. LINC01087 knockdown and the high expression of miR-143-3p inhibited the proliferation, invasion, and EMT processes of TPC-1 and K1 in thyroid cancer cells. LINC01087 negatively targeted miR-135a-5p. Has-miR-135a-5p inhibited the malignant evolution and EMT of thyroid cancer by targeting PPM1E. The PPM1E overexpression can reverse the inhibitory effect of LINC01087 gene knockdown on the proliferation, migration, and invasion of thyroid cancer cells. LINC01087 can promote the proliferation and apoptosis of thyroid cancer cells, and its mechanism may be related to the miR-135a-5p/PPM1E axis.