Role of MicroRNA-223 and MicroRNA-182 as Novel Biomarkers in Early Detection of Colorectal Cancer

Hala A Mahmoud; Hussein Ahmed El Amin; Ehab Saleh Mahmoud Ahmed; Ahmed Gaber Kenawy; Abdallah M El-Ebidi; Islam ElNakeeb; Emad Farah Mohammed Kholef; Wael Abd Elgwad Elsewify

doi:10.2147/IJGM.S353244

Role of MicroRNA-223 and MicroRNA-182 as Novel Biomarkers in Early Detection of Colorectal Cancer

Int J Gen Med. 2022 Mar 25:15:3281-3291. doi: 10.2147/IJGM.S353244. eCollection 2022.

Authors

Hala A Mahmoud¹, Hussein Ahmed El Amin², Ehab Saleh Mahmoud Ahmed¹, Ahmed Gaber Kenawy¹, Abdallah M El-Ebidi³, Islam ElNakeeb⁴, Emad Farah Mohammed Kholef⁴, Wael Abd Elgwad Elsewify¹

Affiliations

¹ Department of Internal Medicine, Faculty of Medicine, Aswan University, Aswan, Egypt.
² Department of Internal Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt.
³ Department of Biochemistry, Faculty of Medicine, Aswan University, Aswan, Egypt.
⁴ Department of Clinical Pathology, Faculty of Medicine, Aswan University, Aswan, Egypt.

Abstract

Background: Colorectal cancer is a common and lethal disease. It is estimated that approximately 145,600 new cases of large bowel cancer are diagnosed annually in the USA. MiRNA-223 and miRNA-182 have been reported in various cancers, such as lung, gastric, breast and colorectal cancer and proposed to be valid and reliable for diagnosis as well as prognosis.

Aim: This study aimed to determine the role of miR-223 and miR-182 as novel biomarkers for early detection and prognosis of CRC.

Patient and methods: This case-control study was conducted at the department of Internal Medicine, Aswan University Hospital, in the period from the 1st of February 2020 to the 20th of April 2021. Thirty-five cases and thirty age- and sex-matched controls were included in the study. All patients were subject to complete clinical evaluation, routine investigations, occult blood in stool, serum levels of CEA and CA 19-9, serum levels of miR-223 and miR-182 by quantitative PCR.

Results: Significant difference between the two studied groups regarding biomarker changes was found. ROC curve analysis showed that the new markers had excellent diagnostic as well as prognostic criteria. Micro-RNA-223 diagnostic accuracy, sensitivity, specificity, PPV, NPV, FDR and FOR were 97%, 97.1%, 96.7%, 97%, 97%, 3.3% and 2.9%, respectively. Also, micro-RNA-182 diagnostic accuracy, sensitivity, specificity, PPV, NPV, FDR and FOR were 97%, 98%, 96%, 96%, 98%, 3.9% and 2%, respectively.

Conclusion: MiR-223 and miR-182 have been discovered to be relevant and reliable biomarkers for the early identification and prognosis of CRC. Increased levels of miR-223 and miR-182 were associated with increased risk of disease progression, and the more accurate the value of miR-223 and miR-182, the earlier the diagnosis of colorectal cancer.

Keywords: CRC; accuracy and prognosis; mirRNA-182; mirRNA-223.