In this paper, we propose a method that optimizes a regularization parameter for the regularized Free Form Deformation (FFD) non-rigid image registration. The developed process utilizes autoencoder generated image representations to assess image data generalization quality by the regularization parameter. Both pixel intensity and learned features are used to improve the overall accuracy and regularity of the resulting inverse problem solution. We implement the new selection criterion with its use in the non-rigid image FFD registration based on multi-level Bspline with L2-regularization, and validate the method with synthetic and real histopathology image datasets. Both qualitative and quantitative results suggest the efficacy of our developed method for fine-tuning histopathology microscope images.
Keywords: Autoencoder; Bspline; Free form deformation; Image registration; Inverse problems; Whole slide image.