Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B

Xiaotong Li; Qunyan Zhu; Fengqin Xu; Minghong Jian; Chaoqun Yao; Hua Zhang; Zhenxin Wang

doi:10.1016/j.ab.2022.114671

Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B

Anal Biochem. 2022 Jul 1:648:114671. doi: 10.1016/j.ab.2022.114671. Epub 2022 Mar 31.

Authors

Xiaotong Li¹, Qunyan Zhu², Fengqin Xu¹, Minghong Jian¹, Chaoqun Yao¹, Hua Zhang², Zhenxin Wang³

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Jinzhai Road Baohe District, Hefei, Anhui, 230026, China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Jinzhai Road Baohe District, Hefei, Anhui, 230026, China. Electronic address: wangzx@ciac.ac.cn.

PMID: 35367218
DOI: 10.1016/j.ab.2022.114671

Abstract

In this work, a lateral flow immunoassay (LFIA) with peptide functionalized gold nanoparticles (termed as biotin-_ppeptide-AuNPs) has been developed for rapid, semi-quantitative detection of PTP1B activity without using any sophisticated equipment. In this method, the anti-phosphotyrosine (anti-pY) monoclonal antibody and streptavidin were used as test line and control line, respectively. The biotin-_ppeptide-AuNPs contain 10% biotinylated peptide ligand carry a motif SDGHEpYIYVDP with pY (phosphotyrosine) and 90% pentapeptide (CALNN) ligand, which are used as PTP1B substrates and LFIA labelling probes. The experimental results demonstrate that the as-proposed LFIA with biotin-_ppeptide-AuNPs exhibits a wide linear range (from 50 ng/mL to 10 μg/mL), a relatively low limit of detection (LOD, 44 ng/mL), and good specificity. In addition, the LFIA with biotin-_ppeptide-AuNPs has been successfully used to evaluate activity levels of PTP1B in four cell lysates and the detection results exhibit a consistent trend with that of commercial kit.

Keywords: Lateral flow immunoassay; Living cell; Peptide functionalized gold nanoparticles; Protein tyrosine phosphatase 1B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotin
Gold*
Immunoassay / methods
Ligands
Limit of Detection
Metal Nanoparticles*
Peptides
Protein Tyrosine Phosphatase, Non-Receptor Type 1

Substances

Ligands
Peptides
Biotin
Gold
Protein Tyrosine Phosphatase, Non-Receptor Type 1