SOD2 rs4880 and GPX1 rs1050450 polymorphisms do not confer risk of COVID-19, but influence inflammation or coagulation parameters in Serbian cohort

Djurdja Jerotic; Jovan Ranin; Zoran Bukumiric; Tatjana Djukic; Vesna Coric; Ana Savic-Radojevic; Nevena Todorovic; Milika Asanin; Marko Ercegovac; Ivana Milosevic; Marija Pljesa-Ercegovac; Goran Stevanovic; Marija Matic; Tatjana Simic

doi:10.1080/13510002.2022.2057707

SOD2 rs4880 and GPX1 rs1050450 polymorphisms do not confer risk of COVID-19, but influence inflammation or coagulation parameters in Serbian cohort

Redox Rep. 2022 Dec;27(1):85-91. doi: 10.1080/13510002.2022.2057707.

Authors

Djurdja Jerotic^{1

2}, Jovan Ranin^{1

3}, Zoran Bukumiric^{1

4}, Tatjana Djukic^{1

2}, Vesna Coric^{1

2}, Ana Savic-Radojevic^{1

2}, Nevena Todorovic³, Milika Asanin^{1

5}, Marko Ercegovac^{1

6}, Ivana Milosevic^{1

3}, Marija Pljesa-Ercegovac^{1

2}, Goran Stevanovic^{1

3}, Marija Matic^{1

2}, Tatjana Simic^{1

2

7}

Affiliations

¹ Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
² Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
³ Clinic of Infectious and Tropical Diseases, Clinical Centre of Serbia, Belgrade, Serbia.
⁴ Institute of Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
⁵ Clinic of Cardiology, Clinical Centre of Serbia, Belgrade, Serbia.
⁶ Clinic of Neurology, Clinical Centre of Serbia, Belgrade, Serbia.
⁷ Serbian Academy of Sciences and Arts, Belgrade, Serbia.

Abstract

Objectives: Due to the role of oxidative stress in the pathophysiology of COVID-19, it is biologically plausible that inter-individual differences in patients' clinical manifestations might be affected by antioxidant genetic profile. The aim of our study was to assess the distribution of antioxidant genetic polymorphisms Nrf2 rs6721961, SOD2 rs4880, GPX1 rs1050450, GPX3 rs8177412, and GSTP1 (rs1695 and rs1138272) haplotype in COVID-19 patients and controls, with special emphasis on their association with laboratory biochemical parameters.Methods: The antioxidant genetic polymorphisms were assessed by appropriate PCR methods in 229 COVID-19 patients and 229 matched healthy individuals.Results: Among examined polymorphisms, only GSTP1 haplotype was associated with COVID-19 risk (p = 0.009). Polymorphisms of SOD2 and GPX1 influenced COVID-19 patients' laboratory biochemical profile: SOD2*Val allele was associated with increased levels of fibrinogen (p = 0.040) and ferritin (p = 0.033), whereas GPX1*Leu allele was associated with D-dimmer (p = 0.009).Discussion: Our findings regarding the influence of SOD2 and GPX1 polymorphisms on inflammation and coagulation parameters might be of clinical importance. If confirmed in larger cohorts, these developments could provide a more personalized approach for better recognition of patients prone to thrombosis and those for the need of targeted antiox-idant therapy.

Keywords: COVID-19; GPX; GSTP1; Nrf2‌; SOD2; inflammation; polymorphisms; thrombosis.

MeSH terms

Blood Coagulation
COVID-19* / enzymology
COVID-19* / genetics
Glutathione Peroxidase* / genetics
Humans
Inflammation / genetics
Polymorphism, Single Nucleotide
Serbia
Superoxide Dismutase* / genetics

Substances

Glutathione Peroxidase
Superoxide Dismutase
superoxide dismutase 2

Grants and funding

This work was supported by the Special Research Program COVID-19, Project No. 7546803 entitled AntioxIdentification, Science Fund of the Republic of Serbia. Program is financed by World Bank loan through Serbia Accelerating Innovation and Entrepreneurship Project – SAIGE.