Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia

Majed H Wakid; Waad T Aldahhasi; Muslimah N Alsulami; Asmaa M El-Kady; Hatem A Elshabrawy

doi:10.2147/IDR.S347220

Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia

Infect Drug Resist. 2022 Feb 17:15:491-501. doi: 10.2147/IDR.S347220. eCollection 2022.

Authors

Majed H Wakid^{1

2}, Waad T Aldahhasi³, Muslimah N Alsulami³, Asmaa M El-Kady⁴, Hatem A Elshabrawy⁵

Affiliations

¹ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
² Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
³ Department of Biology, College of Science, University of Jeddah, Jeddah, 21493, Saudi Arabia.
⁴ Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, 83523, Egypt.
⁵ Department of Molecular and Cellular Biology, College of Osteopathic Medicine, Sam Houston State University, Conroe, TX, 77304, USA.

Abstract

Background: Blastocystis species (sp.) are gastrointestinal protozoan parasites with high prevalence rates worldwide. Blastocystis sp. show extensive genetic diversity with 17 different subtypes (STs) described to date. A few studies have investigated the prevalence and STs of Blastocystis sp. in Makkah, Saudi Arabia. Therefore, we aimed in this study to identify and characterize subtypes of Blastocystis sp. in the City of Makkah, Saudi Arabia.

Methods: Stool samples were collected from 140 patients who presented to King Abdulaziz Hospital, Hera General Hospital and Modern Medical Center in Saudi Arabia. Different microscopic examination methods of patients' stools and molecular analyses (using primers targeting SSU rRNA gene) were performed to identify and characterize STs of Blastocystis sp.

Results: Our microscopic examination of stool samples showed that 96/140 patients (68.6%) had Blastocystis sp. infection. Clinical examination of infected patients revealed that 81 patients were symptomatic, whereas 15 were asymptomatic. Next, we isolated DNA from Blastocystis sp.-positive stool samples followed by PCR amplification of small-subunit ribosomal RNA (SSU rRNA) gene and sequence analysis. Our sequence analysis showed that subtype 3 (ST3) was the most prevalent (53.13%) followed by subtype 1 (ST1) (45.83%), whereas subtype 2 (ST2) was the least prevalent (1.04%). Moreover, our results showed that all three STs resulted in more symptomatic than asymptomatic cases. Finally, we identified novel haplotypes which comprised of 8 ST3, 6 ST1, and one ST2 haplotypes.

Conclusion: Our identification of several haplotypes in patients' stools confirms the genetic diversity of Blastocystis sp. and may explain the reported low host specificity and differential pathogenicity of Blastocystis sp. We believe that additional molecular epidemiological and genomic studies are needed to understand the prevalence and pathogenicity of different subtypes in humans and animal hosts.

Keywords: Blastocystis sp.; ST1; ST2; ST3; Saudi Arabia; genotyping; haplotype; subtype.