Ferroptosis-Related Gene Contributes to Immunity, Stemness and Predicts Prognosis in Glioblastoma Multiforme

Jiawei Dong; Hongtao Zhao; Fang Wang; Jiaqi Jin; Hang Ji; Xiuwei Yan; Nan Wang; Jiheng Zhang; Shaoshan Hu

doi:10.3389/fneur.2022.829926

Ferroptosis-Related Gene Contributes to Immunity, Stemness and Predicts Prognosis in Glioblastoma Multiforme

Front Neurol. 2022 Mar 10:13:829926. doi: 10.3389/fneur.2022.829926. eCollection 2022.

Authors

Jiawei Dong^{1

2}, Hongtao Zhao^{1

2}, Fang Wang^{1

2}, Jiaqi Jin^{1

2}, Hang Ji^{1

2}, Xiuwei Yan^{1

2}, Nan Wang^{1

2}, Jiheng Zhang^{1

2}, Shaoshan Hu^{1

2}

Affiliations

¹ Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
² Cancer Center, Department of Neurosurgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China.

Abstract

Ferroptosis, a recently discovered regulated programmed cell death, is associated with tumorigenesis and progression in glioblastoma. Based on widely recognized ferroptosis-related genes (FRGs), the regulation of ferroptosis patterns and corresponding characteristics of immune infiltration of 516 GBM samples with GSE13041, TCGA-GBM, and CGGA-325 were comprehensively analyzed. Here, we revealed the expression, mutations, and CNV of FRGs in GBM. We identified three distinct regulation patterns of ferroptosis and found the hub genes of immunity and stemness among DEGs in three patterns. A prognostic model was constructed based on five FRGs and verified at the mRNA and protein level. The risk score can not only predict the prognosis but also the degree of immune infiltration and ICB responsiveness by functional annotation. The overall assessment of FRGs in GBM patients will guide the direction of improved research and develop new prognostic prediction tools.

Keywords: ferroptosis; glioblastoma multiforme; immunity; prognosis; stemness.