Collecting and Culturing Bryozoans for Regenerative Studies

Methods Mol Biol. 2022:2450:151-177. doi: 10.1007/978-1-0716-2172-1_8.

Abstract

Among marine invertebrates, bryozoans are small, not well known, and complex to identify. Nevertheless, they offer unique opportunities for whole-body generation research, because of their colonial, modular mode of growth. Here, we describe detailed methods for collection of bryozoans from a range of environments, sample preparation and identification, culture and feeding, spawning and breeding, marking colonies for growth studies, and histological preparation.

Keywords: Anatomy; Brown bodies; Bryozoans; Budding; Collection; Culture; Feeding; Growth; Histology; Larvae; Regression; Settlement; Spawning; Whole-body regeneration.

MeSH terms

  • Animals
  • Aquatic Organisms
  • Bryozoa*