Accurately predicting nitrosylated tyrosine sites using probabilistic sequence information

Abstract

Post-translational modification (PTM) is defined as the enzymatic changes of proteins after the translation process in protein biosynthesis. Nitrotyrosine, which is one of the most important modifications of proteins, is interceded by the active nitrogen molecule. It is known to be associated with different diseases including autoimmune diseases characterized by chronic inflammation and cell damage. Currently, nitrotyrosine sites are identified using experimental approaches which are laborious and costly. In this study, we propose a new machine learning method called PredNitro to accurately predict nitrotyrosine sites. To build PredNitro, we use sequence coupling information from the neighboring amino acids of tyrosine residues along with a support vector machine as our classification technique.Our results demonstrates that PredNitro achieves 98.0% accuracy with more than 0.96 MCC and 0.99 AUC in both 5-fold cross-validation and jackknife cross-validation tests which are significantly better than those reported in previous studies. PredNitro is publicly available as an online predictor at: http://103.99.176.239/PredNitro.

Keywords: Data Imbalance Issue; General PseAAC; Nitrotyrosine Sites Prediction; Post-translational modification; Sequence-coupling Model; Support Vector Machine.