Target Specific Inhibition of Protein Tyrosine Kinase in Conjunction With Cancer and SARS-COV-2 by Olive Nutraceuticals

Arabinda Ghosh; Nobendu Mukerjee; Bhavdeep Sharma; Anushree Pant; Yugal Kishore Mohanta; Rahul D Jawarkar; Ravindrakumar L Bakal; Ermias Mergia Terefe; Gaber El-Saber Batiha; Gomaa Mostafa-Hedeab; Nisreen Khalid Aref Albezrah; Abhijit Dey; Debabrat Baishya

doi:10.3389/fphar.2021.812565

Target Specific Inhibition of Protein Tyrosine Kinase in Conjunction With Cancer and SARS-COV-2 by Olive Nutraceuticals

Front Pharmacol. 2022 Mar 8:12:812565. doi: 10.3389/fphar.2021.812565. eCollection 2021.

Affiliations

¹ Microbiology Division, Department of Botany, Gauhati University, Guwahati, India.
² Department of Microbiology, Ramakrishna Mission Vivekananda Centenary College, Kolkata, India.
³ Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, India.
⁴ Department of Applied Biology, University of Science and Technology Meghalaya, Ri Bhoi, India.
⁵ Department of Medicinal Chemistry, Dr. Rajendra Gode Institute of Pharmacy, Amravati, India.
⁶ Department of Pharmacology and Pharmacognosy, School of Pharmacy and Health Sciences, United states International University-Africa, Nairobi, Kenya.
⁷ Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
⁸ Pharmacology Department & Health Research Unit-Medical College-Jouf University, Sakakah, Saudi Arabia.
⁹ Pharmacology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
¹⁰ Obstetrics and Gynecology Department, College of Medicine, Taif University, Taif, Saudi Arabia.
¹¹ Department of Life Sciences, Presidency University, Kolkata, India.
¹² Department of Bioengineering, Gauhati University, Guwahati, India.

Abstract

The fact that viruses cause human cancer dates back to the early 1980s. By reprogramming cellular signaling pathways, viruses encoded protein that can regulate altered control of cell cycle events. Viruses can interact with a superfamily of membrane bound protein, receptor tyrosine kinase to modulate their activity in order to increase virus entrance into cells and promotion of viral replication within the host. Therefore, our study aimed at screening of inhibitors of tyrosine kinase using natural compounds from olive. Protein tyrosine kinase (PTK) is an important factor for cancer progression and can be linked to coronavirus. It is evident that over expression of Protein tyrosine kinase (PTK) enhance viral endocytosis and proliferation and the use of tyrosine kinase inhibitors reduced the period of infection period. Functional network studies were carried out using two major PTKs viz. Anaplastic lymphoma kinase (ALK) and B-lymphocytic kinase (BTK). They are associated with coronavirus in regulation of cell signaling proteins for cellular processes. We virtually screened for 161 library of natural compounds from olive found overexpressed in ALK and BTK in metastatic as well as virus host cells. We have employed both ligand and target-based approach for drug designing by high throughput screening using Multilinear regression model based QSAR and docking. The QSAR based virtual screening of 161 olive nutraceutical compounds has successfully identified certain new hit; Wedelosin, in which, the descriptor rsa (ratio of molecular surface area to the solvent accessible surface area) plays crucial role in deciding Wedelosin's inhibitory potency. The best-docked olive nutraceuticals further investigated for the stability and effectivity of the BTK and ALK during in 150 ns molecular dynamics and simulation. Post simulation analysis and binding energy estimation in MMGBSA further revealed the intensive potential of the olive nutraceuticals in PTK inhibition. This study is therefore expected to widen the use of nutraceuticals from olive in cancer as well as SARS-CoV2 alternative therapy.

Keywords: ALK; BTK; MD simulation; Olive; QSAR; SARS CoV-2; Wedelosin.