Nitric Oxide Crosstalk With Phytohormone Is Involved in Enhancing Photosynthesis of Tetrastigma hemsleyanum for Photovoltaic Adaptation

Zhuomi Xie; Chuyun Yang; Mingjie Li; Zhongyi Zhang; Yao Wu; Li Gu; Xin Peng

doi:10.3389/fpls.2022.852956

Nitric Oxide Crosstalk With Phytohormone Is Involved in Enhancing Photosynthesis of Tetrastigma hemsleyanum for Photovoltaic Adaptation

Front Plant Sci. 2022 Mar 9:13:852956. doi: 10.3389/fpls.2022.852956. eCollection 2022.

Authors

Zhuomi Xie^{1

2}, Chuyun Yang^{1

2}, Mingjie Li^{1

2}, Zhongyi Zhang^{1

2}, Yao Wu³, Li Gu^{1

2}, Xin Peng^{3

4}

Affiliations

¹ College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.
² Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
³ College of Food Science, Ningbo Research Institute of Zhejiang University, Ningbo, China.
⁴ Medicinal Plant Resource Center, Ningbo Research Institute of Traditional Chinese Medicine, Ningbo, China.

Abstract

Photovoltaic agriculture is a newly emerging ecological planting pattern. In view of the adverse effect on production, a better understanding of photovoltaic adaptation responses is essential for the development of the innovative agriculture mode in sustainable crop production. Here, we investigated the impact of photovoltaic condition on endogenous hormone composition and transcriptome profile of Tetrastigma hemsleyanum. A total of 16 differentially accumulated phytohormones and 12,615 differentially expressed genes (DEGs) were identified. Photovoltaic adaptation significantly decreased the contents of phytohormones especially salicylic acid (SA) and jasmonic acid (JA). DEGs were the most relevant to photosynthesis and mitogen-activated protein kinase (MAPK) signaling pathway especially the key genes encoding proteins involved in photosystem I (PS I) and photosystem II (PS II) reaction center. Nitric oxide (NO), JA, and SA treatment alone significantly enhanced the photosynthetic efficiency which was decreased by exposure to photovoltaic condition, but the combined treatment of "NO + SA" could weaken the enhancement effect by regulating the expression level of psaL, CHIL, petF1, psbQ, and psaE genes. Exogenous phytohormones and NO treatment mitigated the accumulation of reactive oxygen species (ROS) and potentiated antioxidant capacity, which would be weakened by the combined treatment of "NO + SA." SA and JA significantly decreased endogenous NO burst triggered by photovoltaic adaptation. SA might be a potent scavenger of NO and counter the restoration effect of NO on growth and photosynthetic potential in T. hemsleyanum. The results could provide reference for the application of phytohormones/other signaling molecules in photovoltaic agriculture.

Keywords: Tetrastigma hemsleyanum; crosstalk; nitric oxide; photovoltaic adaptation; salicylic acid.