Analysis of protein phosphorylation sites in the hypothalamus tissues of pubescent goats

Xu Yan; Xinbao Gong; Tao Lin; Maosen Lin; Ping Qin; Jing Ye; Hailing Li; Qiwen Hong; Mengxian Li; Ya Liu; Yunsheng Li; Xiujuan Wang; Yunhai Zhang; Yinghui Ling; Hongguo Cao; Xiaorong Zhang; Fugui Fang

doi:10.1016/j.jprot.2022.104574

Analysis of protein phosphorylation sites in the hypothalamus tissues of pubescent goats

J Proteomics. 2022 May 30:260:104574. doi: 10.1016/j.jprot.2022.104574. Epub 2022 Mar 27.

Authors

Xu Yan¹, Xinbao Gong¹, Tao Lin¹, Maosen Lin¹, Ping Qin¹, Jing Ye¹, Hailing Li¹, Qiwen Hong¹, Mengxian Li¹, Ya Liu¹, Yunsheng Li¹, Xiujuan Wang², Yunhai Zhang³, Yinghui Ling³, Hongguo Cao¹, Xiaorong Zhang³, Fugui Fang⁴

Affiliations

¹ Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
² Huoqiu Animal Health Supervision Institute, Animal Husbandry Development Center, Auditorium Road, Huoqiu County, Luan, Anhui 237400, China.
³ Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
⁴ Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: fgfang@ahau.edu.cn.

PMID: 35354085
DOI: 10.1016/j.jprot.2022.104574

Abstract

Protein phosphorylation plays an important role in animal reproduction. However, its role in the onset of puberty in goats remains largely unexplored. Accordingly, in the present study, the molecular changes controlling the onset of puberty in goats were investigated by identifying the differentially phosphorylated proteins (DPPs) and phosphorylation sites (DPSs) in the hypothalami of prepubertal and pubertal female goats using LC-MS/MS and tandem mass tag labelling. A total of 3265 phosphopeptides corresponding to 1628 phosphoproteins were identified, including 234 upregulated and 342 downregulated phosphopeptides. The DPSs HTT, MAP1B, CAMKK1, MAP2, DNAJC5, and GAP43 were identified. These DPPs are enriched in the endocytosis, cAMP signaling, Rap1 signaling, melanogenesis, and insulin secretion pathways. These pathways are related to gonadotropin-releasing hormone and puberty. In particular, glucose-6-phosphate isomerase, fructose-bisphosphate aldolase C, and fructose-bisphosphate aldolase A occupy important locations in the protein-protein interaction network. These data provide evidence for a complex interaction network in goat hypothalamus proteins that affects puberty. Furthermore, they may help identify new puberty-regulating candidates and/or serve as an important resource for exploring the physiological mechanism of puberty onset in mammals. SIGNIFICANCE: This study provides evidence for a complex interaction network in goat hypothalamus proteins that affects puberty. Furthermore, our data may help identify new puberty-regulating candidates and/or serve as an important resource for exploring the physiological mechanism of puberty onset in mammals.

Keywords: Goat; Phosphoproteome; Protein phosphorylation; Puberty.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, Liquid
Female
Fructose-Bisphosphate Aldolase / metabolism
Goats* / metabolism
Hypothalamus / metabolism
Phosphopeptides* / metabolism
Phosphorylation
Tandem Mass Spectrometry

Substances

Phosphopeptides
Fructose-Bisphosphate Aldolase