In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata

Caiping Huang; Jerôme Mutterer; Manfred Heinlein

doi:10.1007/978-1-0716-2132-5_9

In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata

Methods Mol Biol. 2022:2457:151-165. doi: 10.1007/978-1-0716-2132-5_9.

Authors

Caiping Huang¹, Jerôme Mutterer¹, Manfred Heinlein²

Affiliations

¹ Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France.
² Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France. manfred.heinlein@ibmp-cnrs.unistra.fr.

PMID: 35349138
DOI: 10.1007/978-1-0716-2132-5_9

Abstract

The deposition and turnover of callose (beta-1,3 glucan polymer) in the cell wall surrounding the neck regions of plasmodesmata (PD) controls the cell-to-cell diffusion rate of molecules and, therefore, plays an important role in the regulation of intercellular communication in plants.Here we describe a simple and fast in vivo staining procedure for the imaging and quantification of callose at PD. We also introduce calloseQuant, a plug-in for semiautomated image analysis and non-biased quantification of callose levels at PD using ImageJ.

Keywords: Aniline blue; Callose; Confocal microscopy; ImageJ; Plasmodesmata; flg22.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aniline Compounds
Glucans* / analysis
Plasmodesmata* / chemistry
Staining and Labeling

Substances

Aniline Compounds
Glucans
aniline blue
callose