A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines

Aida Amador-Álvarez; María A Gómez-Muñoz; Ismael Rodríguez-Prieto; Ricardo Pardal; Francisco M Vega

doi:10.1016/j.xpro.2022.101260

A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines

STAR Protoc. 2022 Mar 23;3(2):101260. doi: 10.1016/j.xpro.2022.101260. eCollection 2022 Jun 17.

Authors

Aida Amador-Álvarez^{1

2}, María A Gómez-Muñoz^{2

3}, Ismael Rodríguez-Prieto², Ricardo Pardal^{2

3}, Francisco M Vega^{1

2}

Affiliations

¹ Department of Cell Biology, Faculty of Biology, Universidad de Sevilla, 41012 Sevilla, Spain.
² Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain.
³ Department of Medical Physiology and Biophysics, Universidad de Sevilla, 41012 Sevilla, Spain.

Abstract

The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Single Cell; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods
Cell Line
Cell Separation
Cells, Cultured
Humans
Neuroblastoma* / genetics